离子对流动相离子色谱法紫外检测蔬菜中百草枯  

Determination of paraquat in vegetable by ion chromoatography with UV detection

作　　者：费栋[1] 陈梅兰[2] 

机构地区：[1]桂林工学院材料与化学工程系,广西桂林541004 [2]浙江树人大学生物与环境工程学院,浙江杭州310015

出　　处：《环境污染与防治》2008年第6期31-33,共3页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No20375035、No20527005);浙江省自然科学基金资助项目(NoZ404105)

摘　　要：采用离子对试剂作流动相,离子色谱紫外检测法测定蔬菜中残留的百草枯,在流速为0.25mL/min,淋洗液为10%(体积分数)乙腈和0.05mol/L七氟丁酸的混合液时能够快速稳定出峰,且与其他干扰离子充分分离,百草枯的检测下限为5.1μg/L,具有良好的线性关系和重现性。对青菜样品中百草枯进行测定,回收率在86.5%～93.8%,结果令人满意。An ion-pair chromatography-UV detection （at 257 nm） method was developed for separation and measurement of the residual herbicide paraquat in vegetable samples. Rapid separation was achieved in a Dionex IonPac NSI（4 mm× 150 mm） column using 0.25 mL/min of the eluent mixture of 0.05 mol/L HFBA and 10% ACN. Both the peak height and peak area were well correlated with the paraquat concentration by linear functions. With a detection limit of 5. 1 μg/L and 86. 5%-93.8% recovery of the spiked paraquat, the method can be employed to measure residual paraquat in many vegetables and other food items.

关 键 词：离子色谱 紫外检测 百草枯 

分 类 号：R155[医药卫生—营养与食品卫生学]