健脾解毒化浊方对肠易激综合征作用机制的实验研究  

Experiment study on mechanism of Jianpijieduhuazhuofang on irritable bowel syndrome

作　　者：赵玉斌[1] 李佃贵[1] 

机构地区：[1]河北医科大学

出　　处：《现代中西医结合杂志》2008年第20期3091-3094,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家中医药管理局中医药科学技术研究专项计划(No.04-05JP09);河北省自然科学基金资助课题(C2006000988)

摘　　要：目的探讨健脾解毒化浊方对内脏高敏性肠易激综合征(IBS)大鼠模型的疗效和作用机制。方法采用结肠慢性刺激法制作内脏高敏感性的IBS大鼠模型。将实验动物分为正常对照组(A组)、模型对照组(B组)、西药对照组(C组)、中药低剂量组(D组)、中药高剂量组(E组)。观察各组大鼠肠道内扩张引起腹部隆起和背部拱起的容量阈值和大鼠肠道内不同容量下扩张期间腹壁收缩次数,检测5-羟色胺(5-HT)、P物质(SP)、降钙素基因相关肽(CGRP)含量。结果与对照组相比,各治疗组大鼠行为学和电生理指标均有明显改善;各治疗组大鼠5-HT、SP含量明显下降,CGRP含量增加,且有一定的量效关系。结论健脾解毒化浊方能降低内脏高敏感性IBS模型大鼠血清5-HT、血浆SP含量,增加CGRP含量,大剂量痛泻药方疗效优于得舒特。健脾解毒化浊方的作用机制可能是通过降低模型大鼠血清5-HT、血浆SP含量,减弱背角神经元兴奋性,从而达到提高内脏痛阈、消除肠道过敏的治疗目的。Objective It is to discuss the curative effect and mechanism of Jianpijieduhuazhuofang on rat model of internal organs hyperreaction irritable bowel syndrome （IBS）. Methods The rat models of internal organs hyperreaction IBS were established with chronic stimulation in colon. The experiment animals were divided into normal control group, model control group, western control group, low dose traditional Chinese medicine group and high dose traditional Chinese medicine group. The capacity limens that caused abdomen-uplifting and back-arching in intestinal tract inner ectasia of rats in the groups and the contraction time of abdominal wall at ectasia duration under different capacity in rats intestinal tract were observed. The contents of 5 - hydroxytryptamine （5 - HT）, substance P （SP） and calcitonin gene related peptide （CGRP） were detected. Results Compared with control groups, the indexes of ethology and electrophysiology in all treatment groups had obvious improvement. The contents of 5 - HT and SP in all treatment groups were obviously lowered and the content of CGRP was increased, and which has determinate dose-effect relationship. Conclusion Jianpijieduhuazhuofang can lower the contents of serum 5 - HT and blood SP and increase the CGRP content of internal organs hyperreaction IBS rat model. The curative effect of large dose Tongxieyaofang outstrips that of Dicetel. The mechanism of Jianpijieduhuazhuofang may be heightening the pain threshold of internal organs and eliminating hypersusceptibility of intestinal tract through lowering the contents of serum 5 - FIT and blood SP in model rats and decreasing dorsal horn neuronal excitability.

关 键 词：健脾解毒化浊方 肠易激综合征 5-羟色胺 P物质 降钙素基因相关肽 

分 类 号：R-332[医药卫生]