Bt抗性和敏感棉铃虫幼虫中肠主要蛋白酶活性的变化  

Changes in Mid-gut Protease Activities in Larvae of Bt Resistant and Susceptible Strains of Helicoverpa armigera Hübner

作　　者：史艳霞[1,2] 张永军[1] 王桂荣[1] 梁革梅[1] 高继国[3] 吴孔明[1] 郭予元[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 [2]东北农业大学生命科学院哈尔滨150030 [3]东北农业大学生命科学院

出　　处：《应用与环境生物学报》2008年第3期394-398,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点基础研究发展规划项目(No.2007CB109202);国家高技术研究发展计划(2006AA02Z189)~~

摘　　要：采用酶特异性底物测定了3龄和5龄Bt抗性/敏感棉铃虫品系幼虫中肠蛋白酶活性的最适pH值.结果表明,3龄和5龄Bt抗性/敏感棉铃虫幼虫中肠总蛋白酶活性最适pH值均为10.5;强碱性类胰蛋白酶活性最适pH值除3龄Bt抗性幼虫为8.0外,其余均为10.5;弱碱性类胰蛋白酶活性的最适pH值为8.0;类胰凝乳蛋白酶的最适pH值除5龄Bt抗性幼虫的为8.0外,其余都为8.5.比较了最适pH值条件下,3龄和5龄Bt抗性/敏感棉铃虫幼虫中肠蛋白酶活性的差异.发现3龄和5龄Bt抗性/敏感棉铃虫幼虫中肠总蛋白酶活力差异不显著;两个品系3龄幼虫中肠强碱性类胰蛋白酶活性差异达到极显著水平,5龄幼虫中肠强碱性类胰蛋白酶活性差异不明显;两个品系3龄幼虫中肠弱碱性类胰蛋白酶活力差异没有达到显著水平,而5龄幼虫弱碱性类胰蛋白酶活力差异极显著;另外,3龄和5龄Bt抗性/敏感棉铃虫幼虫类胰凝乳蛋白酶活力差异极显著.棉铃虫幼虫中肠酸碱强度和某些蛋白酶活性的变化,可能是造成棉铃虫抗性演化的因素之一.The optimum pH value of mid-gut protease in larvae of both Bt resistant and susceptible strains of Helicoverpa armigera Hubner was determined in laboratory. The results showed that the optimum pH values of total protease in the both strains were 10.5. The optimum pH values of active alkaline trypsin-like enzyme in the two strains were 10.5 except that of the third instar larvae of resistant 1t. armigera was 8.0, while the optimum pH values of weak alkaline trypsin-like enzyme in the strains were 8.0. However, the optimum pH values of chymotrypsin-like enzyme in the two strains were 8.5 except that of the fifth instar larvae of resistant H. armigera was 8.0. The activities of mid-gut protease in both resistant and susceptible strains under the optimum pH condition were also compared. There was no significant difference in the activities of total midgut protease of the two strains. It was shown that the activities of active alkaline trypsin-like enzyme in the fifth instar larvae of the two strains were not significantly different, while those of the third instar larvae of the two strains were significantly different. It was also found that there was no difference in the weak alkaline trypsin-like enzyme activities of the third instar larvae between the two strains, and those of the fifth instar of the two strains were significantly different. As for chymotrypsin- like enzyme, it was found that there was a significant difference in the activities of the two strains. It was concluded that the changes in pH values and mid-gut protease activities could bring out resistance evolution of H. armigera larvae to Bt insecticidal proteins.

关 键 词：棉铃虫 Bt抗性 酶活性 PH值 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]