高效毛细管电泳法测定兽用注射用青霉素钾的含量  

Determination of Penicillin Potassium Salt Injection for Veterinaries by High Performance Capillary Electrophoresis

作　　者：孟欢欢[1] 何东旭[1] 陈玎玎[2] 祁克宗[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽农业大学生物技术中心,合肥230036

出　　处：《中国兽药杂志》2008年第6期29-31,共3页Chinese Journal of Veterinary Drug

基　　金：十一五国家科技支撑计划(2006BAK02A08-4)

摘　　要：研究测定兽用注射用青霉素钾含量的高效毛细管电泳方法,优化了高效毛细管电泳法检测青霉素的电泳条件,缓冲液为pH7.8（6mol/LHCL调节）的30mmol/L硼砂溶液,在分离电压为18kV、温度为25℃条件下青霉素峰形尖锐,浓度与峰面积在40-200μg/mL范围内线性良好,相关系数为0.9971,回收率范围为99.3%-101.5%,日内及日间变异系数均小于4%。This paper has studied the method of determining the content of penicillin potassium salt injection for veterinaries by High Performance Capillary Electrophoresis. The condition of high performance capillary electrophoresis was optimized, the buffer was 30 mmol/L sodium tetraborate adjusted to pH 7.8 with 6 mol/L hydrochloric acid, running voltage was 18 kV and operated temperature was 25 ℃. In this condition peak shape was good, the liner range was 40 - 200 μg./mL and the coefficient was 0. 9971. The recoveries were between 99.3% and 101.5%. The relative standard deviation（RSD） of within day and between day were all less than 4%.

关 键 词：毛细管电泳 青霉素 

分 类 号：S859.796[农业科学—临床兽医学]