TMV重组外壳蛋白Sc1754CP的原核表达和多克隆抗体制备  

Expression of Sc1754CP and Preparation of Anti-Sc1754CP Antibody

作　　者：李蓉[1] 宋任涛[1] 许政暟[1] 李平[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《上海大学学报（自然科学版）》2008年第3期323-327,共5页Journal of Shanghai University:Natural Science Edition

基　　金：上海市科委重点基础研究项目(06JC14030);上海市教委一般项目(06AZ052)

摘　　要：烟草花叶病毒(tobacco mosaic virus,TMV)重组外壳蛋白(coat protein,CP)Sc1754CP是一种能够激发敏感烟草产生过敏性反应(hypersensitive reaction,HR)的蛋白,但是这种蛋白无法通过植物病毒的大量扩增获得.为了获得足够量的上述蛋白,并对这种蛋白在植物中激发的HR反应进行深入研究,作者在大肠杆菌中大量表达Sc1754CP蛋白,并且获得了Sc1754CP高度专一性的多克隆抗体.上述研究为进一步深入研究Sc1754CP及其激发的新的过敏性反应机制奠定了基础.The recombinant coat protein of TMVSc1754 can induce hypersensitive reaction in susceptible tobacco. However this protein hardly is accumulated in ihe inoculated plants. In order to clarify the role of Sc1754CP in this hypersensitive reaction, we over-expressed Sc1754CP in E. coli. Polyclonal Anti- Sc1754CP rabbit polyclonal antibody has been successfully prepared. The Sc1754CP protein and the antibody against Sc1754CP can help us further study this unknown incompatible reaction between pathogen and host.

关 键 词：Sc1754CP 原核表达 多克隆抗体 

分 类 号：Q785[生物学—分子生物学]