中华眼镜蛇毒F组分抗血小板聚集的作用机制  

Inhibitory mechanism of fraction F of Naja naja atra venom on platelet aggregation

作　　者：张梅[1] 余清声[1] 李卫[2] 覃媛[1] 黄劭[1] 孔天翰[1] 

机构地区：[1]广州医学院广州蛇毒研究所,510182 [2]暨南大学第一附属医院神经外科

出　　处：《中华生物医学工程杂志》2007年第5期269-271,共3页Chinese Journal of Biomedical Engineering

基　　金：广州市高校科技计划项目（1015）;广州医学院科技计划项目（03-k-28）

摘　　要：目的探讨中华眼镜蛇毒F组分抑制血小板聚集的作用机制。方法用比浊法测定中华眼镜蛇毒F组分对二磷酸腺苷、花生四烯酸和血小板活化因子诱导血小板聚集作用的影响，流式细胞术观察中华眼镜蛇毒F组分对荧光标记的单克隆抗体CD41（FITC—CD41）和CD61（FITC—CD61）与血小板膜糖蛋白Ⅱb／Ⅲa（GPⅡb／Ⅲa）结合的影响。结果中华眼镜蛇毒F组分明显抑制二磷酸腺苷、花生四烯酸和血小板活化因子诱导的血小板聚集，其作用呈现一定程度的剂量依赖关系。中华眼镜蛇毒F组分可以明显降低单克隆抗体CD41（抗GPⅡb）与血小板的结合率，而对单克隆抗体CD61（抗GPⅢa）与血小板的结合率没有影响。结论中华眼镜蛇毒F组分可以抑制多种激动剂诱导的血小板聚集，其机制和中华眼镜蛇毒F组分与血小板膜糖蛋白Ⅱb／Ⅲa复合物的结合有关。Objective To explore the inhibitory mechanism of fraction F of Naja naja atra venom on platelet aggregation. Methods Nephelometery was used to detect the effect of fraction F of Naja naja atra venom on platelet aggregation induced by adenosine diphosphate （ADP） ,arachidonic acid （AA） and platelet activating factor （PAF）. The influence of fraction F of Naja naja atra venom on the binding of fluoreacenceconjugated monoclonal antibody CIMI （FITC-CIMI） and CD61 （FITC-CD61） to membrane glycoprotein Ⅱb/Ⅲa （GPⅡb/Ⅲa） of platelet was measured by flow cytometry. Results Fraction F of Naja naja atra venom significantly inhibited the aggregation of platelet induced by ADP, AA and PAF in a dose-dependent manner. Fraction F of Naja naja atra venom obviously decreased the binding of FITC-CD41 to GPIIb ,and had no effect on the binding of FITC-CD61 to GPⅢa. Conclusion Fraction F of Naja naja atra venom can inhibit the platelet aggregation induced by different agonists, which is one of mechanisms underlying fraction F of Naja naja atra venom binding to GPⅡb/Ⅲa of platelet.

关 键 词：眼镜蛇毒液类 血小板聚集 血小板糖蛋白Ⅱb/Ⅲa复合物 

分 类 号：R[医药卫生]