重组人p53腺病毒注射液对人淋巴瘤细胞的作用及辐射敏感性的影响  

Effect of recombinant adenovirus encoding human p53 tumor suppressor gene （rAd-p53） on the growth and radiotherapeutic sensitivity of human lymphoma cell lines

作　　者：俞泽阳[1] 范我[1] 吴锦昌[2] 李栋庆[1] 朱然[1] 王永青[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院基础核医学教研室,215123 [2]苏州市立医院放疗科

出　　处：《中华放射医学与防护杂志》2008年第3期213-216,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30471974）

摘　　要：目的探讨重组人p53腺病毒注射液对人淋巴瘤细胞的抑制作用及其辐射增敏性。方法利用四氮唑盐（MTT）比色法分析重组人p53腺病毒注射液、放射治疗以及两者联合应用对人淋巴瘤细胞Raji和Daudi的抑制作用；通过蛋白质的Western印迹分析法（Westernblotting）分析淋巴瘤细胞内P53蛋白的表达；应用反转录．聚合酶链反应（RT-PCR）检测淋巴瘤细胞内p53基因的mRNA表达。结果MTT结果显示重组人p53腺病毒注射液对人淋巴瘤细胞具有一定的抑制作用，但抑制作用不显著，Saji及Daudi细胞最大剂量的抑制率分别为27．5％±4．1％和28．1％±1．6％，也未见明显的辐射增敏效应。Westernblotting和RT-PCR结果表明，外源性P53蛋白和p53基因的mRNA均有所表达，但未见高效表达和明显的辐射增敏性。结论重组人D53腺病毒注射液对人淋巴瘤细胞的抑制作用及辐射增敏性不显著。Objective To explore the inhibitory effect and radiation sensitization of recombinant adenovirus encoding human p53 tumor suppressor gene （rAd-p53） on human lymphoma cell lines. Methods Human lymphoma cell lines Raji and Daudi were treated with rAd-p53, radiation therapy and combined treatment, respectively. The cell growth inhibition was assessed by MTT. The p53 protein expression was detected by Western blotting, and p53 mRNA was detected by RT-PCR. Results The MTT results showed that the inhibitory effect and radiosensitivity enhancement of rAd-p53 on human lymphoma cell lines were not obvious [Rail： （27.5 ±4.1 ）% ; Daudi： （28.1± 1.6） % ]. The results of Western blotting and RT-PCR showed that extrinsic p53 protein and p53 mRNA were expressed to some degree, but not at high-level. In addition, the results didn＇t demonstrate obvious radiosensitivity enhancement. Conclusions The role of inhibition and radiosensitivity enhancement of rAd-p53 was not significant on human lymphoma cell lines.

关 键 词：重组人P53腺病毒注射液 P53基因 人淋巴瘤细胞 放射治疗 

分 类 号：R733.1[医药卫生—肿瘤]