低剂量辐射增强大剂量辐射对人胶质瘤的抑瘤作用  

Low dose radiation enhance the anti-tumor effect of high dose radiation on human glioma cell U251

作　　者：王畅[1] 王冠军[1] 谭业辉[1] 姜宏宇[1] 李薇[1] 

机构地区：[1]吉林大学第一医院肿瘤中心,长春130021

出　　处：《中华放射医学与防护杂志》2008年第3期221-224,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30370439）

摘　　要：目的探讨低剂量辐射及低剂量联合大剂量辐射对人胶质瘤生长的影响。方法以胶质瘤细胞株U251及荷人胶质瘤裸鼠移植瘤为研究对象，分别给予低剂量辐射和低剂量联合大剂量辐射，采用细胞计数、噻唑蓝（MTT）、流式细胞术等方法检测对细胞增殖的影响，通过计算抑瘤率来观察低剂量辐射联合大剂量辐射对移植瘤生长的影响。结果低剂量辐射后U251细胞计数、MTT及流式细胞术检测结果与假照组相比差异无统计学意义；大剂量辐射组及低剂量联合大剂量辐射组细胞增殖明显受抑，凋亡增多，细胞周期G2期阻滞，两组间差异无统计学意义；低剂量辐射联合大剂量辐射组与单独大剂量辐射组均对裸鼠移植瘤有明显的抑制作用，但联合照射组抑瘤作用更强；联合照射组与单纯大剂量辐射组相比，血液系统损伤有所减轻。低剂量辐射组与假照组相比肿瘤生长差异无统计学意义。结论低剂量辐射对人胶质瘤细胞无直接的抑制作用、兴奋效应，不诱导对大剂量辐射适应性反应，但能够在对正常造血系统产生保护作用的基础上，增强大剂量辐射对荷瘤裸鼠移植瘤的抑瘤作用，这种协同抗肿瘤作用在去除细胞免疫影响后仍然存在。Objective To detect the effect on the growth of human glioma cell U251 induced by low dose irradiation and low dose irradiation combined with large dose irradiation. Methods Human glioma cell line U251 and nude mice carried with human glioma were used. The tumor cells and the mice were treated with low dose, high dose, and low dose combined high dose radiation. Cells growth curve, MTY and flow cytometry were used to detect the proliferation, cell cycle and apoptosis of the cells; and the tumor inhibition rate was used to assess the growth of tumor in vivo. Results After low dose irradiation, there, was no difference between experimental group and control group in cell count, MTT and flow cytometry. Single high dose group and low dose combined high dose group beth show significanly the suppressing effect on tumor cells, the apoptosis increased and there was cell cycle blocked in G2 period, but there was no difference between two groups. In vivo apparent anti-tumor effect in high dose radiation group and the combining group was observed, and that was more significant in the combining group; the prior low dose radiation alleviated the injury of hematological system. There was no difference between single low dose radiation group and control. Conclusions There is no significant effect on human glioma cell induced by low dose radiation, and low dose radiation could not induce adaptive response. But in vivo experience, low dose radiation could enhance the anti-tumor effect of high dose radiation and alleviated the injury of hematological system.

关 键 词：低剂量辐射 人胶质瘤细胞 荷瘤裸鼠 抗肿瘤作用 

分 类 号：R739.4[医药卫生—肿瘤]