雌激素对蓝光及过氧化氢诱导的ARPE-19细胞内活性氧生成的影响  

Effects of estrogen on ROS generation induced by blue light and hydrogen peroxide in human retinal epithelial cell ARPE-19

作　　者：雷少波[1] 唐罗生[1] 朱晓华[1] 段宣初[1] 

机构地区：[1]中南大学湘雅二医院眼科,中国湖南省长沙市410011

出　　处：《国际眼科杂志》2008年第6期1133-1136,共4页International Eye Science

摘　　要：目的:观察17-β雌二醇(E2)对蓝光及过氧化氢(H2O2)诱导的人视网膜色素上皮ARPE-19细胞内活性氧(reactive oxygen species,ROS)生成的影响方法:对培养的ARPE-19细胞予以浓度为10nmol/L,100nmol/L,1μmol/L的E2预处理2h后接受蓝光照射(470±20nm,2000±500lx)或200μmol/LH2O2处理,在处理前、处理后30min;1,2,4h行2′,7′-二氢二氯荧光黄双乙酸钠(DCFH-DA)细胞内ROS荧光染色,以490nmol/L荧光显微镜观察照相,收集细胞后以流式细胞仪检测荧光强度。结果:蓝光照射和过氧化氢均可显著增强ARPE-19细胞内的ROS荧光,提示蓝光照射和过氧化氢处理均成功诱导氧化应激,10nmol/L、100nmol/L、1μmol/L17β-雌二醇剂量依赖性地减弱蓝光照射和过氧化氢处理后ARPE-19细胞内的ROS荧光增强的幅度。结论:蓝光照射及过氧化氢处理可诱导ARPE-19细胞内活性氧水平增高;17β-雌二醇可剂量依赖性地抑制蓝光照射诱导的ARPE-19细胞内活性氧水平增高。AIM： To determine whether estrogen suppresses intracellular ROS generation induced by blue light illumination and hydrogen peroxide in human retinal epithelial cell ARPE-19. METHODS： Cultured human retinal epithelial cell line ARPE-19 was pretreated with 17β-estradiol （at concen- tration of 10nmol/L, 100nmol/L, 1μmol/L ） for 2 hours, before given blue light illumination （470 ±20nm,2 000 ±500lx） or 200μmol/L H2 O2 treatment for a variety of time. Intracellular ROS generation was detected by DCFH-DA fluorescent probe and flow cytometry before oxidant treatment and 30 minutes, 1, 2, 4 hours afterward. RESULTS： DCFH-DA fluorescence intensity was significantly increased after blue light illumination or H2O2 treatment, indicating that ROS generation was successfully induced in ARPE-19 cell, the increase in DCFH-DA fluorescence intensity was significantly suppressed by 10nmol/L, 100nmol/L, 1μmol/L 17β-E2 in a dosedependent manner. CONCLUSION： 1713-E2 attenuates blue light and H2 02 induced ROS generation in ARPE-19 cell .

关 键 词：雌激素 视网膜色素上皮细胞 氧化应激 活性氧 光毒性 

分 类 号：R774.1[医药卫生—眼科]