关于单结合胆红素水溶性的研究  

A study on solubility of monoconjugated bilirubin

作　　者：祝学光[1] 涂向群 韩健德[1] 

机构地区：[1]北京医科大学人民医院外科,100044 [2]解放军309医院外科

出　　处：《中华实验外科杂志》1997年第5期289-291,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助课题

摘　　要：目的:进一步研究单结合胆红素(MCB)的水难溶特性及其在病理性胆汁中参与胆红素沉淀和色素性结石形成的作用机制.方法:应用胆红素高效液相色谱分析技术,先从人胆汁中获得结合胆红素,再经分离、制备及纯化得到纯MCB.然后观察其水溶解度并与其它型胆红素比较.结果:在pH7.9,温度37℃接近生理的条件下,MCB的溶解度(558.25±5.96μm)仅为双结合胆红素(DCB)的1/7,比不结合胆红素(UCB)高约44倍并随pH值改变而呈曲线变化.pH7.9时最大;pH4.5时为0;而当pH>9时,反而低于呈离于状态的UCB.结论:MCB虽作为结合型胆红素在胆道生理条件下的不溶性明显高于UCB,但比DCB要低得多;而在胆道病理条件下,其水溶性不但远低于DCB,甚至还不如离子化的UCB,这可能是MCB参与胆红素沉淀及胆色素结石形成的理化基础.Using a newly established HPLC technique, monoconjugated bilirubin (MCB) was extracted, prepared and purified from patients with gallstone and its aqueous solubility was then determined and com pared with disconjugated bilirubin (DCB) and unconju gated bilirubin (UCB). The results showed that in a near physiologic condition (pH 7.9, 37C) of biliary tract, the aqueous solubility of MCB (558.255± 5. 96μm) was only 1/7 of that of DCB (3410.9±2.42μm) but 44 times higher than that of UCB (2.43±0.31μm) (P<0.01, between any two bilirubins). Moreover, the curvilineal change of MCB solubility (highest at pH 7.9, lowest (zero) at pH 4.5) with the alteration of pH, even less than that of ionized UCB at pH above 9, might be the physicochemical basis for MCB in the development of bilirubin precipitation (sludge) and pigment gallstones.

关 键 词：单结合胆红素 溶解度 高效液相色谱法 胆结石 

分 类 号：R575.62[医药卫生—消化系统] R446.129[医药卫生—内科学]