藏花素对膀胱移行细胞癌T24细胞VEGF-C表达水平的影响  

Effect of crocin on the expression of VEGF-C in the human bladder cancer celI lines T24

作　　者：吕纯芳[1] 罗春丽[1] 冀慧莹[1] 赵培[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《重庆医科大学学报》2008年第6期649-651,656,共4页Journal of Chongqing Medical University

基　　金：重庆市科委自然科学基金(No.CSTC2005BB5309)

摘　　要：目的:研究藏花素(Crocin)对膀胱移行细胞癌T24细胞血管内皮生长因子C(Vascular endothelial growth factor,VEGF-C)表达水平的影响。方法:采用MTT法测定不同浓度及不同时间藏花素对T24细胞抑制率,选出最佳药物浓度和最佳作用时间。用RT-PCR和基因芯片分别检测最佳作用时间和最佳药物浓度作用前后T24细胞VEGF-C的表达。结果:MTT显示藏花素抑制T24细胞生长具有时间和剂量依从性,3mmol/L藏花素作用48h对T24细胞的抑制率最高。RT-PCR显示藏花素组VEGF-C表达明显减少,基因芯片扫描结果中藏花素组VEGF-C表达也显著下调。结论:藏花素抑制T24细胞增殖,可能与下调VEGF-C的表达,从而减少肿瘤血管的生成有关。Objective：To study the effect of Crocin on the expression of VEGF-C in transitional cell carcinoma of bladder （TCCB） T24 cell. Methods：T24 cells were treated with Crocin. MTr assay was adopted to determine the inhibition rate for selecting the best effect time and concentration of Crocin. The reverse transcription polymerase chain reaction（RT-PCR） and cDNA microarray were used to evaluate VEGF-C mRNA expression of T24 cells at around the best effect time and as the Crocin concentration reached the best. Results：The growth of T24 cells was inhibited significantly（P〈0.05） at 3mmol/L Crocin when treated for 48h. And there was a marked positive correlation between Crocin concentration, time and inhibition rate. RT-PCR showed that VEGF-C mRNA expression in Crocin treated group was significantly lower than that of the control group. The cDNA microarray analysis was coincided well with the RT-PCR data. Conclusion：Crocin inhibits the proliferation of T24 cells and the mechanism might be decreasing tumor angiogenesis by down-regulating the expression of VEGF-C.

关 键 词：藏花素 膀胱移行细胞癌 表皮生长因子C 

分 类 号：R737.14[医药卫生—肿瘤]