DHAQ-GM-CSF免疫脂质体对HL60细胞的杀伤作用研究  

Effect of DHAQ-GM-CSF immunoliposomes on HL60 cells

作　　者：张颖[1] 娄世峰[1] 

机构地区：[1]重庆医科大学附属第二医院血液科,重庆400010

出　　处：《重庆医科大学学报》2008年第6期673-677,共5页Journal of Chongqing Medical University

摘　　要：目的:制备DHAQ-GM-CSF免疫脂质体并观察其对白血病细胞株HL60的杀伤作用,为探索临床治疗急性髓性白血病(Acute myelogenous leukemia,AML)的新方法提供实验依据。方法:用逆向蒸发法制备DHAQ脂质体、戊二醛交联法偶联GM-CSF和DHAQ脂质体制备GM-CSF免疫脂质体,MTT法测定其对白血病HL60细胞的杀伤作用,透射电镜观察凋亡细胞形态,流式细胞术测定凋亡率。结果:DHAQ-GM-CSF免疫脂质体对HL60的细胞毒作用显著强于DHAQ脂质体和DHAQ,免疫脂质体作用后细胞超微结构破坏,形成大量凋亡小体。结论:成功制备并鉴定了GM-CSF-DHAQ免疫脂质体,其对HL60细胞具有靶向性杀伤作用,为进一步研究奠定了基础。Objective：To prepare DHAQ-GM-CSF immunoliposomes and observe the cytotoxicity of HL60 cells treated with the immunoliposomes for providing the preclinical data for AML therapy. Methods： DHAQ liposomes were prepared by reverse-phase evaporation（REV）,and GM-CSF was coupled to the DHAQ liposomes.The cytotoxicity of HL60 cells treated with the immunoliposomes was measured by tetrazolium microcuhure（MTT） celluar cytotoxicity test.The data of cellar untrastructure and apoptotic rate were collected by transmission electron microscopy,and flow cytometric analysis. Results：DHAQ-GM-CSF immunoliposomes had better effects on the cytotoxicity of HL60 cells than DHAQ liposomes and DHAQ.The cellar untrastructure was markly destroyed and many apoptotic bodies formed after the treatment. Conclusion：DHAQ-GM-CSF immunoliposomes are successfully prepared and verified,which have targeting cytotoxicity on HL60 cells.The study establishes the foundation for futher investigation.

关 键 词：免疫脂质体 免疫导向治疗 白血病 HL60细胞 

分 类 号：R733.7[医药卫生—肿瘤]