硫普罗宁对酒精性肝损伤大鼠的干预作用  

Effect of tiopronin on alcoholic liver injury in rats

作　　者：刘莉[1] 阎明[2] 张喜红[2] 孟繁立[2] 

机构地区：[1]济南市传染病医院肝病二科,山东济南250021 [2]山东大学齐鲁医院消化内科,山东济南250014

出　　处：《现代医药卫生》2008年第14期2063-2065,共3页Journal of Modern Medicine & Health

基　　金：山东省医药卫生科研重点项目资助课题(2007HZ056)

摘　　要：目的:探讨硫普罗宁对大鼠酒精性肝损伤的干预作用。方法:用酒精灌胃建立酒精性肝病大鼠模型,成模后模型组随机分为干预组、未干预组和干预对照组,4周后处死,分别测量肝功生化指标,肝组织巯基和丙二醛含量,光学显微镜下观察肝脏组织病理变化。结果:模型组肝功生化指标与还原性巯基含量均明显异常;硫普罗宁干预后血清肝功能各指标均显著改善,肝组织巯基含量明显增加(P<0.01),丙二醛含量下降(P<0.05),同时肝脏病理变化明显改善。干预对照组指标改善优于硫普罗宁干预组(P<0.01)。结论:还原性巯基减少是酒精性肝病的重要变化,补充还原性巯基可以减轻或改善肝细胞损伤程度,但戒酒仍是首选和重要的干预治疗办法。Objective：To investigate the effect of tiopronin on alcoholic fatty liver disease in rats. Methods：One hundred male Wis- tar rats weighting between 170 g and 190 g were divided into two groups randomizedly：model group and control group.The model group was given alcoholic intragastric administration plus olive oil diet.After 12 weeks of treatment,The rats of model group were divided into three groups randomizedly：intervention treatment group, untreated group and normal sodium group.After 4 weeks, the rats were killed,respectively determinated the hepatic funtion parameters, serum TG,TC, ALT, AST, ALP, GGT, hydrosulfide and MDA in the liver tissue. Liver histological pathology was analyzed under light microscope.Results：Compared with the untreated group,the tiopronin intervention treantment group improved significantly in hepatic funtion parameters and hydrosulfide content, the activity of anti-oxidation enzyme also increased obviously.Meanwhile the pathology was improved significantly. The normal sodium group improved greatly compared with the intervention treantment group （P〈0.01）. Conclusion：Stopping drinking is a basic and important treatment in hastening liver cell recovery. At the same time the intervention of tiopronin plays a role in prevention of alcoholic injury by increasing hydrosulfide content in the body.

关 键 词：酒精性肝病 硫普罗宁 动物模型 干预治疗 

分 类 号：R36[医药卫生—病理学]