低剂量辐射对胚胎鼠神经源干细胞增殖影响的体外实验研究  

Effect of low dose radiation on the proliferation capacity of rats embryonic neural stem cells in vitro

作　　者：刘长江[1] 于春雷[1] 张弘[1] 

机构地区：[1]吉林大学第四医院神经外科,吉林省长春市130011

出　　处：《中国厂矿医学》2008年第3期259-261,I0001,共4页Chinese Medicine of Factory and Mine

摘　　要：目的探讨低剂量辐射(LDR)对胚胎鼠神经源干细胞(NSCs)体外增殖的影响。方法从孕14 d SD大鼠中获得胚胎鼠NSCs并进行体外传代培养,并对LDR前后的细胞进行鉴定,将培养至对数期的细胞分成13组,即0、25、75、200 mGy各剂量照射后4 h、24 h、48 h、72 h,各组细胞分别采用台盼蓝拒染法、四甲基偶氮唑蓝(MTT)实验及流式细胞仪方法检测细胞的增殖程度。结果胚胎鼠NSCs体外培养获得稳定传代的NSCs,在诸多辐射剂量和时间框内,以75 mGy照射后的24 h细胞增殖最明显,细胞的增殖指数(PI)最高,且照射前后细胞的鉴定结果显示LDR不会改变其分化潜能和原始状态。结论LDR体外可以刺激胚胎鼠NSCs的增殖效应。Objective To investigate the effect induced by low dose radiation（LDR） on the proliferation hormesis capacity of rat embryonic neural stem cells（NSCs）. Methods Embryonic NSCs were isolated from pregnancy 14 d SD rats and performed serial subcultivation. The exponential growth stage cells were divided into 13 groups, including 0 mGy LDR group and 4 h, 24 h, 48 h, 72 h groups after 25, 75, 200 mGy LDR. NSCs were identified before and after LDR. The extent of cells proliferation were detected with trypan blue exclusion staining, MTT and flow cytometry methods. Results NSCs were cultured successfully. The proliferation was most obvious when NSCs were cultured con- tinuously for 24 h after 75 mGy LDR, which did not change the primitive state and ability to differentiate to neuron and horizontal cell. Conclusions LDR can stimulate the proliferation potency of embryonic NSCs in vitro.

关 键 词：低剂量辐射 神经源干细胞 细胞增殖 大鼠 胚胎 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R815[医药卫生—基础医学]