逆转录聚合酶链扩增与免疫组化在检测胃癌区域淋巴结微转移中的相关研究  

Study on the Comparison of the Detection Rates of Regional Lymph Node Micro-Metastasis Obtained with Immuno-Histochemistry and RT-PCR

作　　者：张德[1] 李继广[1] 魏明[1] 

机构地区：[1]泰山医学院聊城市第二人民医院检验科PCR实验室,252600

出　　处：《放射免疫学杂志》2008年第3期253-255,共3页Journal of Radioimmanology

摘　　要：目的:利用逆转录聚合酶链扩增检测胃癌常规病理检查阴性的淋巴结微转移的发生及与免疫组化结果的关系。方法:利用逆转录聚合酶链反应(RT-PCR)方法检测480枚胃癌胃周淋巴结癌胚抗原(CEA)mR-NA基因表达,同时比较RT-PCR与免疫组化方法的检测敏感性。结果:利用RT-PCR检测CEA mRNA是一种很敏感的方法。检测138例胃癌患者取材的480枚胃周淋巴结。免疫组化阳性率27.5%(132/480)。RT-PCR阳性率58.8%(282/480),两组之间有显著性差异(P<0.01);RT-PCR阳性率随着病期进展而增大。结论:RT-PCR技术是比免疫组化更敏感的方法,可以预测胃癌病人淋巴结微转移,有效避免已有微小转移的患者被漏诊、误诊。Objective To study the detection rate of micro - metastasis obtained with immuno - histochemistry and RT - PCR respectively in pathologically negative regional lymphnodes in resected gastric cancer specimens. Methods Micrometastasis was detected with immuno - histochemistry and RT - PCR amplication of CEA mRNA gene expression respectively in 480 pathologically negative lympimodes from 138 resected gastric cancer specimens. Results Positive rate was significantly higher with RT - PCR technique （58.8%, 282/480） than that with immuno- histochemistry （27.5%, 132/480） （P〈0.01）. Highter positive rates were found in more advanced cases. Conclusion RT- PCR technic is much more sensitive than the conventional immuno -histochemical method for detection of micro - metastasis in lymphnodes from gastric cancer specimens.

关 键 词：胃癌 RT—PCR 淋巴结 CEAMRNA 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤] R737.31[医药卫生—临床医学]