X射线对肺腺癌细胞E-cadherin/β-catenin及其相关生物学特性的影响  

The effect of X-irradiation on E-cadherin/β-catenin and related biological characteristics of A549 human lung adenocarcinoma cell line

作　　者：蒋幼凡[1] 刘晓[1] 岳秀[1] 雷淑慧[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸内科,重庆400010

出　　处：《肿瘤》2008年第6期476-479,共4页Tumor

基　　金：重庆市卫生局科技项目(编号:03-2-053)

摘　　要：目的:探讨X射线在抑制肺腺癌细胞侵袭和转移中的作用机制。方法:采用6 MV不同剂量X射线照射肺腺癌细胞A549,软琼脂细胞集落形成实验检测细胞增殖抑制;采用RT-PCR和Western印迹法分别从mRNA和蛋白水平检测细胞中E-cadherin及其连环蛋白β-catenin的表达;Transwell小室检测细胞侵袭力。结果:X射线对A549细胞的增殖抑制作用呈剂量依赖性;照射后E-cadherin的总蛋白和mRNA的表达水平与对照组相比差异均有统计学意义(P<0.05),其变化趋势是随照射剂量的增加其表达逐渐增加;但β-catenin在总蛋白和mRNA水平上的变化差异无统计学意义(P>0.05)。随着照射剂量的增加,细胞侵袭力逐渐降低(P<0.05)。结论:X射线可上调A549细胞上黏附分子E-cadherin和β-catenin在细胞中的表达,增加细胞间的黏附力,降低肿瘤细胞的侵袭力,这可能是X射线抑制肺腺癌细胞转移的作用机制之一。Objective：To investigate the mechanism by which X-irradiation inhibited the invasion and metastasis of human lung adenocarcinoma cell line. Methods：A549 cells were irradiated with varying doses of 6 MV X-ray. Soft agar colony formation assay was employed to evaluate the effects of X-irradiation on the proliferation of A549 cells. RT-PCR and Western blotting were performed to analyze the protein and mRNA expression levels of E-cadherin and β-catenin. The invasion of A549 cells was evaluated by the Transwell chamber migration assay. Results：X-irradiation could inhibit the proliferation of A549 cells in a dose-dependent manner. The protein and mRNA expression levels of E-cadherin in A549 cells were higher than those in the control group （ P 〈 0.05 ）. But there were no significant differences in the total protein and mRNA expression levels of β-catenin （ P 〉 0.05 ）. And the cell invasion capability was decreased with the increase in the dose of irradiation （ P 〈 0.05 ）. Conclusion-X-irradiation up-regulated the expression of E-cadherin and β-catenin in A549 cells, which enhanced the intracellular adhesion and suppressed the invasion capability. That may be the mechanism for the inhibitory effects of X-irradiation on metastasis of human lung adenocarcinoma cell line.

关 键 词：癌 非小细胞肺 X线疗法 肿瘤转移 A549细胞 E—cadherin Β-CATENIN 

分 类 号：R734.2[医药卫生—肿瘤] R730.55[医药卫生—临床医学]