异鼠李素与脱氧核糖核酸相互作用的电化学与紫外光谱法研究  

Electrochemical and UV-Vis Spectrophotometric Studies on the Interaction between Isorhamnetin and Deoxyribonucleic Acid

作　　者：林丽清[1] 陈敬华[1] 黄丽英[1] 林新华[1] 

机构地区：[1]福建医科大学药物分析系,福建福州350004

出　　处：《分析测试学报》2008年第6期635-637,640,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20675015);福建省卫生厅青年基金资助项目(2006111)

摘　　要：采用电化学方法,结合紫外-可见光谱法研究异鼠李素(ISOR)在碳糊电极上与DNA的作用机制,并通过天然DNA(fsDNA)和变性DNA(ssDNA)与异鼠李素的作用的不同,得出异鼠李素与DNA分子发生嵌插作用的结论,形成DNA-2[ISOR]1∶2型的超分子化合物,求得结合常数β=1.03×105L/mol。因此ISOR有可能成为一种用于DNA分析测定的新型探针。Isorhamnetin（ISOR） exhibited good electrochemical behavior at carbon paste electrode in acidic medium and could interact with DNA to form a supramolecular complex DNA -2[ ISOR] with a binding constant of 1.03 × 10^5 L/mol. The interaction mechanism was investigated by analyzing the natural DNA（fsDNA ）-ISOR and the denatured DNA（ssDNA）-ISOR with cyclic vohammetry and UV -Vis speetrophotometry. Compared with the results obtained by fsDNA -ISOR, it was found that the peak current and the absorption peak intensity obtained by fsDNA -ISOR reduced only to a small extent, indicating ISOR has intercalated into the fsDNA double helix in the course of interaction. Thus ISOR may be used as a novel probe for the determination of DNA.

关 键 词：异鼠李素 核酸 循环伏安法 紫外-可见分光光度法 

分 类 号：Q52[生物学—生物化学] O657.1[理学—分析化学]