几种新的植物甾醇类衍生物对人类癌细胞系增殖的影响  

INHIBITION BY PHYTOSTEROL DERIVATIVES OF HUMAN CANCER CELL PROLIFERATION

作　　者：李红星[1] 高佳丽[1] 刘彦锋[1] 李淑贤[2] 高芳[1] 翟永功[1] 

机构地区：[1]北京师范大学生命科学学院生物医学研究所、基因工程药物及生物技术北京市重点实验室,北京100875 [2]邯郸学院化学系,河北邯郸056005

出　　处：《北京师范大学学报（自然科学版）》2008年第3期293-298,共6页Journal of Beijing Normal University(Natural Science)

摘　　要：探讨几种新的植物甾醇类衍生物抑制人肝癌细胞系HepG2和人乳腺癌细胞系MCF-7生长及诱导其凋亡的生物学效应.采用形态学观察,溴化二甲噻唑二苯四氮唑(MTT)还原法和流式细胞仪(FCM)检测3种新的植物甾醇衍生物在不同作用时间、处理浓度时,对HepG2和MCF-7细胞生长的影响.实验结果表明:3种新的植物甾醇衍生物对2种细胞系的生长具有明显抑制作用,且呈现一定的对浓度和时间的依赖效应,并具有诱导细胞凋亡的功能.To investigate modulation by several new phytosterol derivatives of human hepatocarcinoma HepG2 and breast cancer MCF-7 cell proliferation in vitro. Light microscopy, MTT assay and flow cytometry were used to examine their induction of apoptosis in HepG2 and MCF-7 cells at varied concentrations for different time periods. All three phytosterol derivatives significantly inhibited HepG2 and MCF-7 cell proliferation in a dose- and time-dependent manner. A typical apoptotic morphology was observed. All three phytosterol derivatives inhibited significantly HepG2 and MCF-7 cell proliferation at least partly by apoptosis.

关 键 词：植物甾醇衍生物 细胞凋亡 流式细胞术 

分 类 号：Q813[生物学—生物工程] TS202.3[轻工技术与工程—食品科学]