异体角膜对人外周血T细胞及其亚群CD_(28)分子表达的体外调节  

The Regulation of Allogeneic Cornea to CD 28 Expression on Human Peripheral Blood T Lymphocytes and Its Subsets in Vitro

作　　者：钟敬祥[1] 徐锦堂[1] 王丽娅[1] 李辰[1] 

机构地区：[1]暨南大学医学院附属医院眼科

出　　处：《眼科研究》1997年第3期155-157,共3页Chinese Ophthalmic Research

摘　　要：目的观察异体角膜体外对人外周血Ｔ细胞及其亚群ＣＤ２８分子表达的调节。方法应用异体角膜与外周血淋巴细胞体外共同培养模型和流式细胞分析技术（ｆｌｕｏｒｅｓｃｅｉｎａｃｔｉｖａｔｅｄｃｅｌｓｏｒｔｅｒ，ＦＡＣＳ）。结果对照组Ｔ细胞ＣＤ２８表达为２１．３％；受角膜或沸波醇酯（ｐｈｏｒｂｏｌ１２，１３—ｄｉｂｕｔｙｒａｔｅ，ＰＤＢ）激活后，Ｔ细胞ＣＤ２８表达分别为５２．４％和８１．４％；受角膜激活后，ＣＤ４和ＣＤ８Ｔ淋巴细胞ＣＤ２８表达分别为６３．１％和５２．３％。结论角膜组织体外能够刺激人外周血Ｔ细胞ＣＤ２８表达和Ｔ细胞活化，ＣＤ４Ｔ细胞比ＣＤ８Ｔ细胞活化更明显。ObjectiveTo observe the immunoregulation of allogeneic cornea to the activation of human peripheral blood T lymphocytes in vitro,and CD 28 expression of T lymphocytes and its subsets when T lymphocytes were stimulated by allogeneic cornea or phorbol 12,13—dibutyrate(PDB).MethodsCulture of human peripheral blood lymphocytes in vitro was studied with allogenic cornea or PDB serving as the stimulative antigen.After co culture for 72 h,T lymphocytes were labelled by monoclonal antibody,and analysed by fluorescein activated cell sorter(FACS).ResultsCD 28 expression on T lymphocytes in control was 21.3%;After stimulation by the allogeneic cornea,CD 28 expression on T lymphocytes was 52.4 %;After stimulated by the PDB,CD 28 expression on T lymphocytes was 81.4%;After stimulated with cornea,CD 28 expression on CD + 4 or CD + 8 subset T lymphocytes was 63.1% or 52.3%.ConclusionAllogeneic cornea can stimulate rather than suppress CD 28 expression on human peripheral blood T lymphocytes in vitro and activation of T lymphocytes.CD 28 can enhance the signal delivery during the activation of T lymphocytes.The activation on CD + 4 T lymphocytes is more prominent than on CD + 8T lymphocytes.

关 键 词：角膜移植 异体移植 T细胞 T细胞亚群 移植免疫 

分 类 号：R779.65[医药卫生—眼科] R392.12[医药卫生—临床医学]