慢性粒细胞性白血病细胞周期蛋白D1基因表达及临床研究  

Study of the expression of the cyclin D1 in chronic myelogenous leukemia

作　　者：尹列芬[1] 杨玲[1] 崔萌萌[1] 申政磊[2] 瞿晓媛[1] 程丽芳[1] 黄桂云[1] 

机构地区：[1]昆明医学院第二附属医院血液科,650101 [2]昆明医学院第三附属医院血液科

出　　处：《白血病．淋巴瘤》2008年第3期187-188,191,共3页Journal of Leukemia & Lymphoma

基　　金：云南省教育厅科学研究资助项目（520315C）

摘　　要：目的检测细胞周期蛋白D1（cyclin D1）在慢性粒细胞性白血病（CML）患者中的表达，探讨cyclin D1在CML中有无异常表达及其表达与病情进展和预后的关系。方法采用荧光实时定量PCR方法检测18例CML和16例良性血液病为对照组cyclin D1的表达。结果CML患者cyclin D1的表达水平高于对照组，差异有统计学意义（P〈0．001）；加速期／急变期cyclin D1的表达水平明显高于慢性期，差异有统计学意义（P〈0．05）。cyclin D1表达的水平在对照组（0．1980）、CML慢性期（1．4002）、加速期／急变期（5．4540）之间呈明显的由低到高的上升趋势。结论cyclin D1在CML中有异常高表达，提示cyclin D1在CML中可能有癌基因的作用，其表达水平与CML患者的病情进展程度及预后有关。Objective To examine the expression levels of cyclin D1 in the patients with chronic myelogenous leukemia （CML）, and evaluate the pathogenesis and clinical significance of cyclin D1 in CML. Methods The real-time quantitative polymerase chain reaction （RQ-PCR） was performed to detect the expression levels of cyclin D1 in the bone marrow samples of 18 patients with CML, and 16 samples of benign hemopoietic patients. The relationship between the expression levels of cyclin D1 and the progression and prognosis of patients with CML were analyzed. Results The level of cyclin D1 was higher expressed in 18 patients with CML than the control group （P 〈0.001）. The levels of cyclin D1 was apparently higher expressed in accelerated phase/blast crisis phase than in chronic phase （P 〈0.05）. And the RQ-PCR method showed the tendency that a significant increase was observed in the levels of cyclin D1 from 0.1980 in control group to 1.4002 in chronic phase and 5.4540 in accelerated phase /blast crisis phase. Conclusion The cyclin D1 overexpressed in CML, the roles of cyclin D1 in CML might be an oncogene expressed. The expression level is correlated with the progression and orognosis of oatients with CML.

关 键 词：白血病 髓样 慢性 细胞周期蛋白D1 荧光实时定量PCR 

分 类 号：R733.72[医药卫生—肿瘤]