反相HPLC法同时测定烟草中的甲酸、乙酸和乳酸  

Simultaneous Determination of Formic,Acetic and Lactic acids in Tobacco by Reversed-phase High Performance Liquid Chromatography

作　　者：王文领 王宁[2] 宋金勇 郝辉 

机构地区：[1]河南中烟工业公司许昌卷烟厂技术中心,河南省许昌市461000 [2]河南大学化学化工学院,河南省开封市475001

出　　处：《烟草科技》2008年第6期41-43,共3页Tobacco Science & Technology

摘　　要：以0.01mol/LNaH2PO4-H3PO4(pH=1.20)缓冲溶液作酸化剂,丙酮为提取剂,对溴苯甲酰甲基溴为衍生化试剂,WatersC18(3.9mm×150mm,5μm)作色谱柱(柱温35℃),乙腈、甲醇和水为流动相(梯度洗脱,流速1.0mL/min),采用反相液相色谱法同时测定(检测波长255nm)了8种烟草样品中的甲酸、乙酸和乳酸。结果表明:①3种有机酸的回收率为97.9%～106.5%;相对标准偏差5.17%～7.33%;检测限1.32～3.30μg/mL;②2种卷烟样品的乙酸含量均与6种烟叶样品的差别不大,而其甲酸和乳酸含量却远远高于烟叶样品,尤其是乳酸含量约是烟叶样品的2～8倍。Using 0.01 mol/L NaH2PO4-H3PO4 （pH1.20）buffer solution as acidulant, acetone as extracting solvent, p-bromophenacyl bromide as deriving agent, the formic, acetic and lactic acids in eight tobacco samples were simultaneously determined by HPLC at the wavelength of 255 nm with Waters C18 column （3.9mm×150mm,5μm） at 35℃ and CH3OH-CH3CN-H2O as mobile phase at the flow rate of 1.0 mL/min. The results showed that：1 ） the recoveries of the three organic acids ranged from 97.9% to 106.5% with the relative standard deviations of 5.17% to 7.33% and the limits of detection of 1.32 to 3.30μg/mL; 2） there was no obviously difference between the acetic acid contents in the two cigarette samples and the six tobacco leaf samples, while the contents of formic and lactic acids in the cigarette samples were far higher, especially, lactic acid content was 2 to 8 times that in the leaf samples.

关 键 词：反相高效液相色谱法 甲酸 乙酸 乳酸 烟草 

分 类 号：TS411.1[农业科学—烟草工业]