重组人内皮抑素两种给药途径抑制角膜新生血管的实验研究  

The inhibitory effect of recombinant human endostatinon to rat corneal neovascularization by two deferent medication ways

作　　者：李维义[1] 高晓唯[2] 郭继华[2] 任兵[2] 

机构地区：[1]石河子大学医学院 [2]解放军第474医院眼科医院,新疆乌鲁木齐830000

出　　处：《农垦医学》2008年第3期185-188,共4页Journal of Nongken Medicine

摘　　要：目的:研究重组人内皮抑素(recombinant human endostatin,rHES,恩度)两种给药途径对碱烧伤诱导大鼠角膜新生血管的抑制作用。方法:48只Wistar大鼠随机分为4组:A组(12只眼),对照组,不做处理;B组(12只眼),角膜碱烧伤后球结膜下注射生理盐水0.05ml,隔日1次;C、D组(各12只眼),右眼角膜碱烧伤后分别应用100mg/ml重组人内皮抑素点眼,4次/日和球结膜下注射0.05ml,隔日1次治疗。观察各组角膜新生血管的生长情况,免疫组化检测VEGF蛋白的表达。结果:C组、D组在碱烧伤后各时间点新生血管面积均小于B组,C组、D组与B组新生血管面积的差异有统计学意义(P<0.05);D组较C组对新生血管的抑制作用略强,差异无统计学意义(P>0.05)。碱烧伤后第16天免疫组织化学检查显示除C组与D组间,其余各两两比较VEGF蛋白量差异有统计学意义,C组与D组VEGF蛋白量差异无统计学意义(P>0.05)。结论:点眼和球结膜下注射重组人内皮抑素(恩度)即均可有效抑制CNV。Objective： To investigate the inhibitory effect of recombinant human endostatin（rHES） on alkalineinduced rat corneal neovascularization by eye drop and subeonjunctival injection. Methods： Corneal neovaseularization animal model was established using 1 mol/L NaOH solution in rat. 48 Wistar rats were divided into 4 groups： A group（ 12eyes）, control groups, normal eyes; B group（ 12eyes）, N.S. were injeeted into subconjunetival tissues one time every other day after alkaline burn; C group（ 12 eyes） and D group（ 12eyes）were respectively treated by rilES（100mg/ml） drops,4times a day and subeonjunctival injection one time every other day. The growth state of CNV was observed, the expression of VEGF in conea was detected with immunohistoehemieal assay, Results：The areas of corneal neovascalarization in C group and D group were significantly lower than that B group at each time point（ P 〈 0. 05）, but there was not significant difference between C group and D group（ P 〉 0. 05） , And VEGF expresion on 16th day is similar. Conclusion： Both eye dropping and subconjunctival injection of recombinant human endostatin can effectively inhibit alkaine- induced corneal neovascularization.

关 键 词：重组人内皮抑素 角膜新生血管 

分 类 号：R772[医药卫生—眼科]