固定化脱氮细菌协同除氨氮废水及生物膜观察  

Ammonia Removal of Wastewater by Immobilized Ammonia-oxidizing Bacteria with Denitrying Bacteria and Biofilm Observation

作　　者：陈君[1] 张艾晓[1] 钟卫鸿[1] 陈建孟[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《生物技术》2008年第3期76-80,共5页Biotechnology

基　　金：国家自然科学基金项目资助(20276070);浙江省自然科学基金项目资助(Y304091)

摘　　要：联合利用本实验室分离得到的亚硝酸菌Ochrobactrum anthropi CZ和好氧反硝化菌Alcaligenes faecalis CZ处理模拟氨氮废水。O.anthropi CZ在7d内将50mg/L的初始氨氮氧化成亚硝氮,平均速率为0.28mg/L·h。A.faecalis CZ在有氧条件下,于2d内将200mg/L的初始硝态氮完全降解,平均降解速率为4.17mg/L·h。分别将两种菌用海藻酸钙(CA)包埋固定化、聚氨酯泡沫(PUF)固定化、海藻酸钙吸附生物膜和游离法处理模拟氨氮废水。结果发现,各种固定化细胞的降解能力明显比游离细胞强。其中CA包埋细胞法效果最好,可以在24h内将23mg/L初始氨氮完全降解。表面吸附生物膜方法也显示了良好的除氮能力,通过核酸染料染色,在荧光显微镜下进行了两种细菌在CA表面的生物膜表征方法的探索。Two strains of nitrogen removal bacteria were isolated and combined to treat ammonium wastewater. Ochrobactnan anthropi CZ oxidated 50mg/L of initial ammonia to nitrite with nitrification rate of 0.28mg/L·h. Alcaligenes faecalis CZ deoxidized 200mg/L of initial nitrate in two days with denitrification rate of 4.17 mg/L. h under aerobic condition. Calcium alginate （CA） gel beads and polyurethane foam （PUF） were used to compare the ability in nitrogen removal between planktoinc cells and different immobilized cells. Bacteria embedded in CA beads showed the highest competence since in this group, 23mg/L initial ammonium nitrogen could be removed in 24h in aerobic circumstance. Bacteria attached on the CA bead also showed good ability to remove nitrogen. We also studied and observed the biofilm of the two strains under fluorescence microscopy after being dyed bv a kind of nucleic acid dyestuff.

关 键 词：亚硝酸菌 好氧反硝化菌 固定化 海藻酸钙 聚氨酯泡沫 生物膜 

分 类 号：X172[环境科学与工程—环境科学]