核糖体相关基因在大鼠再生肝及肝肿瘤中表达异同的比较  

THE SIMILARITIES AND DIFFERENCES IN EXPRESSION OF RIBOSOME-RELATED GENES BETWEEN RAT REGENERATION LIVER AND LIVER TUMOR

作　　者：徐存拴 杨志利[2] 董华明[2] 

机构地区：[1]河南师范大学生命科学学院 [2]河南省-科技部共建细胞分化调控重点实验室,新乡453007

出　　处：《解剖学报》2008年第3期281-287,共7页Acta Anatomica Sinica

基　　金：国家973计划前期研究专项基金资助项目(2006CB708506)

摘　　要：目的在基因转录水平了解核糖体相关基因在大鼠再生肝(RL)和肝脏肿瘤(LT)中的表达异同。方法用搜索网站资料和查阅相关论文等方法获得核糖体发生、组装和功能相关基因及它们在肝脏肿瘤中的表达变化,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用统计学方法比较上述基因在再生肝和肝脏肿瘤中的表达异同。结果初步证实上述基因中75个基因与肝再生相关。其中,18个基因在再生肝和肝脏肿瘤中表达上调,6个基因在两者中表达下调,51个基因在肝脏肿瘤中未发生有意义的表达变化。结论再生肝的核糖体相关基因表达谱与肝脏肿瘤有相同之处,也有不同之处,前者的基因表达更具阶段性和复杂性。Objective To address the expression correlation of ribosome-related genes in rat regeneration liver （RL） and those in liver tumor at transcriptional level. Methods Genes involved in ribosome biogenesis, assembly and function, as well as their expression changes in liver tumor, were obtained by online data collection and literature review, and their expression profiles in rat regenerating liver were detected by Rat Genome 230 2.0 array. Expression similarities and differences of the above genes were analyzed using statistical methods. Results A total of 75 genes were found to be RL-related. In regenerated liver and liver tumor, the expressions of 18 genes increased and 6 genes decreased, and no significant expression changes were found in 51 genes in liver tumor. Conclusion The expression profiles of the ribosome-related genes in regenerated liver cells and liver tumor cells have both similarities and differences, and the former exhibit properties of more gradualness and complexity.

关 键 词：部分肝切除 核糖体 肝再生相关基因 基因组2302.0芯片 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]