脂类代谢和运输涉及的基因与大鼠肝再生的相关性分析  

RELEVANCE OF LIPID METABOLISM AND TRANSPORT-INVOLVED GENES TO RAT LIVER REGENERATION

作　　者：徐存拴 蔺芳[2] 秦少伟[2] 

机构地区：[1]河南师范大学生命科学学院 [2]河南省-科技部共建细胞分化调控重点实验室,新乡453007

出　　处：《解剖学报》2008年第3期302-309,共8页Acta Anatomica Sinica

基　　金：国家973计划前期研究专项资助项目(2006CB708506)

摘　　要：目的在基因转录水平了解脂类代谢和运输相关基因在大鼠肝再生（LR）中的表达变化和模式。方法用搜集网站资料和查阅相关论文等方法获得参与脂类代谢和运输基因,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用比较手术和假手术中基因表达的差异性确定肝再生相关基因。结果初步证实上述基因中193个基因与肝再生相关。肝再生早期[部分肝切除（PH）后0.5～4h]、前期（PH后6～12h）、中期（PH后12～66h）、后期（PH后72～168h）等4个阶段起始表达的基因数为113、20、66和1;基因的总表达次数为250、205、796和293。共上调852次,下调630次,分为27种表达方式。肝再生早期和前期胆汁酸代谢相关基因转录减弱;早期和后期糖皮质激素分解相关基因转录增强;前期和中期磷脂合成相关基因转录增强,磷脂分解相关基因转录减弱;中期脂肪酸、白三烯和鞘糖脂合成相关基因转录增强,甘油三酯和磷脂酰肌醇代谢相关基因转录增强,鞘糖脂分解相关基因转录减弱;中期和后期前列腺素合成和脂肪酸分解相关基因转录增强;几乎在整个肝再生中性激素、糖皮质激素和孕酮合成相关基因转录增强,鞘磷脂代谢相关基因转录增强,脂类运输相关基因转录增强,胆固醇代谢相关基因转录减弱。结论肝再生中脂类代谢和运输变化较大,与肝再生密切相关。Objective To realize the expression changes and patterns of lipid metabolism and transport-related genes in rat liver regeneration （LR） at transcriptional level. Methods Genes involved in lipid metabolism and transport were obtained by retrieving the databases data and the related papers. The gene expression changes during LR was checked by Rat Genome 230 2.0 array, and LR-associated genes were identified by comparing gene expression difference between partial hepatectomy （PH） and sham operation （SO） groups. Results One hundred ninety three genes were found to be LR-associated. The number of initially and totally expressed genes occurring in forepart（0.5-4 hours after PH） of LR, prophase（6-12 hours after PH）, metaphase（ 12-66 hours after PH）, and anaphase（72-168 hours after PH） was 113, 20, 66, 1 and 250, 205, 796, 293, respectively. The number of their up-regulation and down-regulation was 852 and 630 times, and their expression profiles were classified into 27 types. The subdued transcription levels occur to the bile acid metabolism-involved genes in forepart and prophase of LR; the elevated transcription levels occur to the glucocorticoid catabolism-involved genes in forepart and anaphase ; phospholipids synthesis-involved genes were up-regulated in prophase and metaphase, while phospholipids catabolism-involved genes were down-expressed; fatty acid, leukotriene and glycosphingolipid synthesis-involved genes, triglyceride and phosphatidylinositol metabolism-involved genes were up-regulated in metaphase, while glycosphingolipid catabolism-involved genes were down-expressed; the elevated transcription levels occur to the prostaglandin synthesis and fatty acid catabolism-involved genes in metaphase and anaphase; during almost the whole period of LR, genes participating in sex hormone, glucocorticoid and progesterone synthesis, sphingomyelin metabolism and lipid transport were up-regulated, while cholesterol metabolism-involved genes were down- expressed. Conclusion Lipid metabolism and

关 键 词：部分肝切除 脂类代谢 运输相关基因 肝再生相关基因 基因组2302.0芯片 大鼠 

分 类 号：R364.2[医药卫生—病理学]