8Hz次声作用对大鼠海马脑源性神经营养因子表达的影响  

The effect of 8 Hz infrasound on the expression of brain derived neurotrophic factor in the rat hippocampus

作　　者：袁华[1] 龙华[2] 牟翔[1] 李玲[3] 唐晨[1] 张美霞[1] 葛雪松[1] 刘静[1] 瞿丽莉[1] 陈景藻[1] 

机构地区：[1]第四军医大学西京医院康复与理疗科,西安710032 [2]第四军医大学唐都医院骨科 [3]北京中国人民解放军总医院第一附属医院康复理疗科

出　　处：《中华物理医学与康复杂志》2008年第5期309-312,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：全军医学科学技术研究“十五”计划指令性课题（01L071）

摘　　要：目的研究频率为8Hz，声压级分别为90，100和130dB的次声作用对大鼠海马脑源性神经营养因子（BDNF）表达水平的影响。方法将48只sD大鼠随机分为对照组和90，100和130dB次声作用组（次声频率均为8Hz），每组12只。将各次声作用组大鼠暴露于8Hz、不同声压级次声环境中，次声每天作用2h；对照组大鼠同期也置于次声舱内，但期间不给予次声干预。于实验进行4周后，将各组大鼠处死取脑，采用免疫印迹法（Westernblot）检测大鼠海马中BDNF蛋白含量变化情况；采用原位杂交法检测BDNFmRNA在海马分布中的变化。结果各次声作用组大鼠海马中BDNF含量均有不同程度减少，随着次声声压级提高，BDNF水平下降幅度逐渐加重。原位杂交结果显示BDNFmRNA在大鼠海马各区域中均有分布，各次声作用组大鼠海马BDNFmRNA水平均较对照组下降，其中以齿状回部位的下降幅度最为显著。结论实验大鼠经频率为8Hz，声压级为90，100或130dB的次声作用后，其海马（尤其是齿状回区）BDNF含量减少，BDNFmRNA表达水平下降，这可能是次声作用引起机体学习记忆障碍的重要原因之一。Objective To investigate the effect of 8 Hz infrasound on the expression of brain derived neurotrophic factor （BDNF） in the rat hippocampus. Methods Forty-eight Sprague-Dawley rats were randomly divided into groups to be exposed to 90, 100 and 130 dB infrasound, and a control group （ n = 12）. All the animals in the in- frasound exposure groups were exposed to 8 Hz infrasound at the planned intensity for 2 hours daily for 4 weeks. The rats of the control group were treated identically except that the infrasound amplitude was 0 dB. The animals were sacrificed and their brains were examined at the end of the 4-week infrasound exposure. Western blotting was used to de- tect the expression of BDNF protein, and in situ hybridization （ISH） was used to observe the distribution of BDNF mRNA in the hippocampus. Results Eight Hz infrasound induced down-regulation of BDNF protein in the hippo- campus, with volume-dependent characteristics. ISH showed that BDNF mRNA was distributed widely in the hippocampus. After exposed to infrasound, BDNF mRNA in the hippocampus decreased, especially in the dental gyrus. Conclusion Eight Hz infrasound can down-regulate the expression of BDNF in the hippocampus, especially in the dental gyrus.

关 键 词：次声 海马 脑源性神经营养因子 

分 类 号：R741[医药卫生—神经病学与精神病学]