高压氧对缺血再灌注小鼠脑组织MMP-9、MMP-2mRNA的表达及血脑屏障通透性的影响  

Effects of hyperbaric oxygen on the expression of MMP-9 and MMP-2 mRNA in the brain tissues and on the permeability of the blood brain barrier after cerebral ischemia and reperfusion

作　　者：赵红[1] 朱丽娜[1] 武剑华[2] 张海鹏[3] 陈学新[4] 

机构地区：[1]中国医科大学基础医学院机能实验中心,沈阳110001 [2]中国医科大学91期七年制学生 [3]中国医科大学病理生理教研室,沈阳110001 [4]中国医科大学附属第一临床医院高压氧科

出　　处：《中华物理医学与康复杂志》2008年第5期313-315,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：辽宁省教育厅高校科研基金资助项目（2005L456）

摘　　要：目的探讨高压氧（HBO）对缺血再灌注小鼠脑组织中基质金属蛋白酶（MMPs）MMP-9、MMP-2mRNA的表达及血脑屏障通透性的影响。方法复制清醒小鼠脑缺血再灌注模型，并于再灌注期间行0．25MPaHBO治疗5次，在处死动物前1h经尾静脉注射2％伊文蓝（EB），采用逆转录多聚酶链反应（RT—PCR）方法及比色法分别检测脑组织中MMP-9、MMP-2mRNA的表达及EB的含量。结果脑缺血再灌注组MMP-9、MMP-2mRNA的表达及EB的含量明显高于假手术组，差异有统计学意义（P〈0．01），高压氧组与假手术组比较，脑组织中MMP-9、MMP-2mRNA的表达及EB的含量相近（P〉0．05），HBO＋脑缺血再灌注组脑组织中基质金属蛋白酶MMP-9、MMP-2mRNA的表达及EB的含量明显低于脑缺血再灌注组，差异有统计学意义（P〈0．01）。结论高压氧可明显减少脑缺血再灌注脑组织中基质金属白蛋酶MMP-9、MMP-2的表达，具有降低血脑屏障通透性的作用。Objective To investigate the effects of hyperbaric oxygen （HBO） on the expression of MMP-9 and MMP-2 mRNA in the brain tissues after cerebral ischemia and reperfusion, and the permeability of blood brain barrier （BBB）. Methods Using cerebral isehemia-reperfusion models with conscious mice, 0.25 MPa （atmospha- era absolutus,ATA） HBO was applied 5 times during the reperfusion period, and 2% Evan＇s blue （EB） was injected into the tail vein 1 hour before the animals were sacrificed. The expression of MMP-9 and MMP-2 mRNA and EB content were determined by RT-PCR and spectrophotometry. Results The expression of MMP-9 and MMP-2 mRNA as well as EB content significantly increased in the ischemia-reperfusion group as compared with a sham surgery group. The expression of MMP-9 and MMP-2 mRNA, and EB levels in the HBO group were similar to those in the sham sur- gery group. The expression of MMP-9 and MMP-2 mRNA and EB levels in the group given HBO plus reperfusion group were significantly decreased as compared with those in the group receiving reperfusion alone. Conclusion HBO can significantly reduce the expression of MMP-9 and MMP-2 mRNA and the permeability of the BBB.

关 键 词：高压氧 血脑屏障 缺血再灌注 基质金属白蛋酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]