HIF-1α介导肝癌血管生成的多层CT灌注研究  

Multi-slice CT Perfusion Study on Angiogenesis in Hepatocellular Carcinoma Induced by Hypoxia Inducible Factor-1α

作　　者：解云川[1] 周翔平[2] 

机构地区：[1]泸州医学院附属医院放射科,四川泸州646000 [2]四川大学华西医院放射科

出　　处：《实用放射学杂志》2008年第6期759-763,共5页Journal of Practical Radiology

摘　　要：目的研究多层CT灌注与缺氧诱导因子-1α（HIF-1α）介导肝癌血管生成的相关性及临床意义。方法手术、病理证实的34例肝癌、11例肝血管瘤行多层CT灌注成像，获取参数。免疫组化SP法检测34例肝癌、11例肝血管瘤及周围正常肝中缺氧诱导因子-1α、血管内皮生长因子（VEGF）的表达，CD34单克隆抗体标记微血管密度，将两者对比研究。结果肝癌的肝动脉灌注量（HAP）、肝动脉灌注指数（HAI）均高于肝血管瘤及周围正常肝（P〈0．01）。肝癌缺氧诱导因子-1α与VEGF及CD34的表达呈正相关（P〈0．01）。肝癌HAP和HPI与HIF-1α、VEGF及CD34的表达呈正相关（P〈0．05）。肝癌的Edmondson—Steiner组织学分级从Ⅰ级、Ⅱ一Ⅲ级、Ⅳ级间HAP、HAI、MVD的差异有统计学意义（P〈0．05）。结论多层CT灌注在一定程度上反映HIF-1α介导的缺氧条件下的肝癌血管生成。Objective To study the correlation between multi - slice CT perfusion imaging and angiogenesis in hepatocellular carcinoma（ HCC） induced by hypoxia inducible factor - 1α（ HIF - 1 α）. Methods Multi - slice CT perfusion data were obtained in 34 cases of HCC and 11 cases of hepatic hemangioma identified by operation and pathology. Positive expression of HIF - 1α and vascular endothelial growth factor（ VEGF） protein of the lesions in all cases and surrounding normal hepatic tissue were investigated with SP immunohistochemistry. Microvessel density （MVD） was determined by anti - CD34 immunostaining. The correlation hetween multi - slice CT peffusion data and positive expression of HIF - 1α,VEGF and CD34 was investigated simultaneously. Results Hepatic arterial perfusion （HAP） and hepatic arterial perfusion index（HAI） in HCC were higher than that of hepatic hemangioma and surrounding normal hepatic tissue（P 〈0.01 ）. HIF -1α was positively correlated with VEGF and CD341P 〈 0.01） in HCC. HAP and HPI were positively correlated with HIF - 1α, VEGF and CD34 （P 〈0.05） in HCC. There was statistical significance between the differences among positive expression of HIF -1α, VEGF, CD34 and multi - slice CT peffusion data in Edmondson - Steiner grading （ P 〈0.05） of HCC. Conclusion CT peffusion can reflect the angiogenesis in HCC induced by HIF - 1α to some extent.

关 键 词：癌 肝细胞 体层摄影术 X线计算机 缺氧诱导因子-1Α 

分 类 号：R735.7[医药卫生—肿瘤] R814.42[医药卫生—临床医学]