乳腺癌树突状细胞瘤苗对自体CIK细胞生物活性的影响  

Effects of dendritic cellular tumor vaccine of breast cancer on bilogical activity of autologous CIK cells

作　　者：何劲松[1] 郭良峰[1] 陈伟财[1] 王敏[1] 吴恢升[1] 王媛[1] 王先明[1] 刘仁斌[2] 

机构地区：[1]广东省深圳市第二人民医院乳腺外科,518035 [2]中山大学附属第一医院普外科,广州51010

出　　处：《岭南现代临床外科》2008年第3期173-176,共4页Lingnan Modern Clinics in Surgery

基　　金：2007年广东省科技计划项目(No:73125)

摘　　要：目的探讨乳腺癌树突状细胞瘤苗(DCs)对自体CIK细胞生物活性的影响。方法取诱导7d的活化DCs瘤苗和诱导7d的自体CIK细胞混合培养(DC-CIK细胞),同期自体CIK细胞为对照组。检测2、8、14d的DC-CIK细胞CD3与CD56表型、培养上清液IL-12的水平及测定混合培养8d时对乳腺癌细胞MCF-7的细胞毒效应。结果混合培养14d的DC-CIK细胞增殖率显著高于CIK细胞(P<0.01),但CD3和CD56表型无显著变化(P>0.05)。DC-CIK细胞对MCF-7乳腺癌细胞的特异性溶解率和细胞毒活性均高于CIK细胞(P<0.01);CIK细胞强烈刺激DCs分泌IL-12,混合培养2d时,DCs的IL-12p40分泌水平达到高峰。结论乳腺癌DCs瘤苗与自体CIK细胞混合培养后(DC-CIK细胞),进一步促进了DCs的成熟并增强了CIK细胞的细胞毒活性。Objective To investigate the effects of dendritic cellular tumor vaccine on the biological actvity of autologous CIK cells. Methods The induced 7 days activated DCs tumor vaccine and autogenous CIK cells were taken for mixed culture. The autogenous CIK cells were served as control group at the same period. CD3 and CD56 phenotype of DC-CIK cells and IL-12 level of cultured supernatant fluid were determinated on induced 2,8 and 14 days. And cytotoxic effect of MCF-7 of breast cancer cells were detected during 8 days mixed culture. Results In the 14 days of mixed culture, reproduction rates of CIk cells were significanhy higher than that CIK cells（P〈0.01）. But CD3 and CD56 phenotype were no obvious change（P 〉 0.05）. In the specific dissolution rate and cytotoxic activity of MCF-7 of breast cancer cells, the DC-CIK cells were higher than that CIK cells （P〈0.01）. Because of CIK cells could irritate DCs to secrate IL-12,the secreting level of IL-12 P40 of DCs was reached to high peak during 2 days of mixed culture. Conclusion After mixed culture of breast cancer DCs tumor vaccine and autogenous CIK cells, it would promote further maturity of DCs and enhance the cytotoxic activity of CIK.

关 键 词：乳腺癌 树突状细胞 瘤苗 细胞因子激活的杀伤细胞 

分 类 号：R737.9[医药卫生—肿瘤]