纳豆激酶体外活性测定及影响因素考察  

Determination of Nattokinase activity in vitro and investigation on influential factors

作　　者：王怡鑫[1] 尹宗宁[1] 张霞[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《中国生化药物杂志》2008年第3期168-171,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金资助项目(30371696)

摘　　要：目的建立纳豆激酶（NK）体外活性测定方法，考察影响因素。方法采用对甲基苯磺酰-L-精氨酸甲酯（TAME）作为底物，用变色酸反应显色，在574nm波长处比色测定酶活性。结果NK在0．05—0．25mg／mL浓度范围内具有良好的线性关系；NK在pH8．0，4℃，活性保持最久。结论TAME法测定NK活性操作简便，可较准确测定NK体外活性。温度、pH、某些溶剂对NK的活性有影响。Purpose To establish a method for determining Nattokinase activity in vitro, and to investigate the influential factors. Methods Na-4-tosyl-L-arginine methyl ester hydrochloride（TAME）is used as the substrate of Nattokinase, colored by the chromotropic acid, determined in 574 nm by UV. Results Data between the Nattokinase content and absorbance had a better linear relationship in the range 0.05-0.25 mg/mL（ r = 0.998 5）.The Nattokinase activity maintains longer at pH 8.0 and 4℃. Conclusion The TAME method is simple, and it has been applied successfully to determine Nattokinase activity in vitro. Temperature, pH and solvent have influence on Nattokinase activity.

关 键 词：纳豆激酶 甲基苯磺酰-L-精氨酸甲酯 活性测定 

分 类 号：TQ460.7[化学工程—制药化工]