氩氦冷冻治疗系统对犬脑冷冻范围的超导刀直径变量和时间相关性研究  

Effects of superconducting knife diameter and freezing time of Endocare CryocareTM Surgical System on frozen necrosis area and histopathological changes of normal canine brain

作　　者：陈强[1] 卢凤飞[1] 张世忠[1] 王雪涛[1] 李明[1] 

机构地区：[1]南方医科大学珠江医院神经外科,广东神经外科研究所,广州510282

出　　处：《中华神经医学杂志》2008年第6期592-595,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨氩氦冷冻治疗系统（简称氩氦刀）在超导刀直径及冷冻时间变量下，正常犬脑组织冷冻坏死范围及超微组织病理学变化。方法用直径2mm、3mm氩氦冷冻超导刀插入犬脑一侧右额叶，分别冷冻3min、5min，重复一次，48h后灌注取脑组织，在光镜和电镜下观察组织病理学变化，测量冷冻坏死区直径。结果冷冻后48h，冷冻区脑组织均呈出血坏死状，可明显分为中央坏死区、炎性反应带、出血水肿带和交界线，冷冻边缘未发现存活的脑细胞结构。结论采取2次冻融循环，冷冻时间为3min、5min，足以导致冷冻区脑组织死亡。Objective To investigate the effects of the variables of superconducting knife diameter and freezing time of Endocare CryocareTM surgical system on the necrosis extent and micro-pathological changes of the normal frozen brain tissue of dogs. Methods Argon-helitm refrigeration superconducting knives of 2 and 3 mm in diameter were respectively inserted into the right frontal lobe of dog brains, and the brains were frozen for 3 and 5 min, respectively. The procedure was repeated one time. 48 h later, the brains were perfused and harvested for the pathological observation under light microscope and electron microscope and the determination of necrosis area. Results 48 h after refrigeration, the frozen brains presented hemorrhagic necrosis and were obviously divided into the central necrosis, inflammatory reaction zone, bleeding and edema zone. The boundaries were clear. Survival of brain cell structure was not found in the edge of frozen area. Conclusion Two freeze-thaw cycles with the freezing time of 3 and 5 min are enough to cause the death of frozen brain tissue.

关 键 词：氩氦刀 脑组织 组织学 病理学 

分 类 号：R739.4[医药卫生—肿瘤]