特发性血小板减少性紫癜患者CD4～+CD25～+Treg细胞的表达  

Expression of CD4～+CD25～+regulatory T cells in patients with idiopathic thrombocytopenic purpura

作　　者：沈权[1] 司今[1] 俞立权[1] 金哈斯[1] 

机构地区：[1]武警总医院血液科,北京市100039

出　　处：《中国组织工程研究与临床康复》2008年第25期4895-4898,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:近年研究发现,CD4+CD25+Treg细胞不论数量缺失或功能紊乱均可导致免疫异常的发生,对调节性T细胞的深入认识,将会对免疫异常疾病如自身免疫性疾病、移植物抗宿主病、过敏性疾病等发病机制的揭示有着深远的意义。实验将进一步验证CD4+CD25+Treg细胞与特发性血小板减少性紫癜发病的关系及可能机制。方法:实验于2007-06/09在中国医学科学院、中国协和医科大学血液学研究所实验室完成。①实验材料:中国协和医科大学血液病医院住院的特发性血小板减少性紫癜患者27例治疗前后外周血,患者确诊符合血液病诊断及疗效标准。正常人外周血15份,实验均征得患者或家属知情同意。②实验方法:应用流式细胞术检测特发性血小板减少性紫癜患者、正常对照、以及治疗前后患者外周血中CD4+CD25+Treg细胞的比例。对其中治疗有效的23例患者,采用密度梯度离心法从治疗前后的外周血中提取单个核细胞,以RT-PCR方法检测其中Foxp3 mRNA的表达水平。结果:①特发性血小板减少性紫癜患者外周血中CD4+CD25+Treg细胞的比例以及CD4+CD25+Treg细胞在总CD4+T细胞中所占比例明显均低于正常对照组(P<0.05)。②有效治疗后的特发性血小板减少性紫癜患者CD4+CD25+Treg细胞的比例与治疗前相比无明显改变(P>0.05),但Treg细胞中Foxp3 mRNA的表达水平比治疗前明显增加(P<0.05)。结论:特发性血小板紫瘢患者体内存在CD4+CD25+Treg细胞的异常,数量的减少、Foxp3表达的降低所造成的CD4+CD25+Treg细胞免疫抑制功能受损可能是特发性血小板减少性紫瘢发病的一个重要因素。AIM： It has been recently proved that either decreased number or impaired function of CD4^＋CD25^＋regulatory T cells （Treg） can lead to immune dysfunction. Deeper knowledge of CD4^＋CD25^＋ Treg cells is crucial for a better understanding of the pathogenesis of many diseases, such as autoimmune diseases, graft-versus -host disease, and allergic disease. This study is aimed to investigate the correlation of CD4^＋CD25^＋ Treg cells in patients with idiopathic thrombocytopenic purpura （ITP） and to analyze the possible immunopathogenesis. METHODS： The experiment was conducted in the laboratory of Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College from June to September in 2007. After the patients and their relative signed informed consents, peripheral blood was obtained from ITP patients （n=27） and health people （n=15） admitted in the Hematology Hospital, Peking Union Medical College. All patients accorded with the standards of ITP diagnosis and curative effect. The amount of CD4^＋CD25^＋ Treg cells in peripheral blood of normal controls and 1TP patients before and after therapy was detected by flow cytometry. For those 23 patients who received effective therapy, blood before and after therapy were both obtained, the mononuclear cells （MNCs） were isolated from peripheral blood by density gradient centrifugation, then reverse transcriptase polymerase chain reaction （RT-PCR） was used to analyze the expression of Foxp3 mRNA. RESULTS： The amount of CD4^＋CD25^＋ Treg cells and the ratio of CD4^＋CD25^＋ Treg to total CD4^＋ T cells in peripheral blood of ITP patients were obviously decreased than those of the controls （P 〈 0.05）. After effective therapy, the amount of CD4^＋CD25^＋ Treg cells in peripheral blood of ITP patients were not changed statistically （P 〉 0.05）, but the expression of Foxp3 mRNA in CD4^＋CD25^＋ Treg cells was significantly increased （P 〈 0.05）. CONCLUSION： Abnormality of CD4^＋C

关 键 词：调解性T细胞 RT—PCR FOXP3 流式细胞仪 细胞工程 

分 类 号：R394.2[医药卫生—医学遗传学]