镉对鸡垂体Fas和caspase-3 mRNA表达的影响  

Effect of cadmium on the expression of Fas and caspase-3 mRNA in chicken anterior pituitary

作　　者：何宝霞[1] 傅业全[2] 李金龙[1] 徐世文[1] 

机构地区：[1]东北农业大学动物医学院,哈尔滨150030 [2]周口职业技术学院动物科学系,周口466001

出　　处：《环境科学学报》2008年第7期1419-1424,共6页Acta Scientiae Circumstantiae

基　　金：黑龙江省教育厅资助项目(No10551038)~~

摘　　要：探讨氯化镉(CdCl2)诱导鸡脑垂体细胞凋亡的发生情况及对凋亡相关基因Fas和caspase-3表达的影响.选择健康50日龄海兰白蛋鸡90只,分为3组,每组30只,以CdCl2含量为0、140、210mg·kg-1的拌料饲喂,分别于染毒20d、40d和60d时取垂体,用原位末端标记(Tunel)和RT-PCR方法检测细胞凋亡和基因表达情况.实验结果表明,CdCl2作用后可致鸡腺垂体细胞呈现明显的凋亡征象,并可诱导垂体细胞Fas和caspase-3mRNA表达的增加;在整个试验期的各个时间点2个剂量组的凋亡率和基因表达量与对照组相比差异显著(p<0.01),且随着染镉时间的延长,低剂量组的凋亡率以及Fas、caspase-3 mRNA表达增加较明显.这表明,一定剂量的CdCl2可诱导鸡垂体细胞凋亡,在此过程中Fas和caspase-3 mRNA表达呈现出与凋亡较为一致的趋势.The effect of cadmium chloride on cell apoptosis and the expressions of Fas and caspase-3 genes were explored in a hen model, Ninety normal Hyline white laying hens, aged 50 days, were randomly allocated to 3 groups of 30. The hens were fed 0 mg·kg^-1 CdCl2 in the control group, 140 mg·kg^-1 CdCl2in the low-dosage cadmium group and 210 mg·kg^-1 CdCl2in the high-dosage cadmium group. The anterior pituitary glands of the hens were removed after 20 d, 40 d and 60 d and the levels of cell apoptosis were detected by TUNEL and the expressions of Fas and caspase-3 genes were measured by RT-PCR. The results indicated that cadmium chloride can lead to apoptosis of pituicytes and overexpression of Fas and caspase-3 genes. At the same time point, cell apoptosis rates and gene expression showed variance （p〈 0.01） in both test groups compared with control and the low-dosage group changed greatly both the expression of Fas, caspase-3 mRNA and cell apoptosis rates. The data indicated that cadmium chloride could induce apoptosis of anterior pituitary cells. The expression of Fas and caspase-3 mRNA have the same trend as apoptosis with the extension of time.

关 键 词：镉 鸡垂体 凋亡 FAS CASPASE-3 

分 类 号：X171.5[环境科学与工程—环境科学]