VEGF对白血病树突状细胞诱导CTL杀伤活性的影响  

Effects of vascular endothelial growth factor on dendritic cell induced CTL activation

作　　者：李维[1] 杨松[2] 张伶[2] 涂植光[2] 

机构地区：[1]重庆市血液中心,重庆400015 [2]重庆医科大学医学检验系教育部临床检验诊断学重点实验室

出　　处：《中国输血杂志》2008年第5期342-345,共4页Chinese Journal of Blood Transfusion

基　　金：重庆市教委科学技术研究项目(编号:KJ050309)

摘　　要：目的观察血管内皮生长因子(VEGF)对白血病树突状细胞(DC)激活的CTL体外杀伤活性的影响。方法K562白血病细胞经5μmol/L VEGF反义寡核苷酸作用24 h后诱导生成DC。流式细胞术检测DC特征性表型(CD40、CD86、HLA-DR和CD83)的表达,MTT法检测DC激发的细胞毒T淋巴细胞(CTL)对靶细胞的杀伤活性。ELISA法检测DC上清中IL-12的表达。结果与正常K562诱生的DC(K562-DC)相比,K562经反义寡核苷酸下调VEGF表达后培养出具有典型特征的DC(AS-K562-DC),它不仅高表达DC免疫表型,而且激活的CTL对K562细胞具有更强的杀伤效应,同时具有更强的IL-12分泌能力(P均<0.05)。结论抑制白血病细胞VEGF表达,能够促进白血病源DC的分化和成熟,激活的CTL在体外具有更强的抗白血病效应。Objective To investigate the effects of vascular endothelial growth factor （VEGF） on cytotoxlc T lymphocyte （CTL） activation induced by dendritic cells （DC）, generated from K562 leukemic cell lines in vitro. Methods After treatment by vascular endothelial growth factor （ VEGF ） antisense phosphorothioate oligodeoxynucleotide （ODN） for 24 h, the K562 cells were cultured with cytokines to differentiate into DC. The expression of DC phenotypes were assayed by flow cytometry. The tumoricidalactivity of CTL primed by DC was measured by MTT assay. The concen-trations of IL-12 were detected by ELISA in the supcmatant of cultured DC. Results The VEGF treated K562 cells were induced into AS-K562-DC, and demonstrated distinctive morphological features of DC and high expression of pheno- types. Furthermore, cytotoxicities by CTL derived from AS-K562-DC and the capability of secreting IL-12 by AS-K562- DC were stronger than K562-DC control （P〈0.05）. Conclusion Inhibiting YEGF can improve the maturation of leu-kemic DC. CTL induced by DC have stronger cytotoxic activities on leukemic cells in vitro.

关 键 词：血管内皮生长因子 树突状细胞 白血病 细胞毒性T淋巴细胞 

分 类 号：R730.51[医药卫生—肿瘤] R733.7[医药卫生—临床医学]