胃癌组织中CyclinD1与P27蛋白表达及其意义  

EXPRESSION OF CyclinD1 AND P27 AND THEIR CLINICAL SIGNIFICANCE IN GASTRIC CARCINOMA

作　　者：高涵[1] 于建宪[1] 

机构地区：[1]青岛大学医学院附属青岛市市立医院病理科,山东青岛266071

出　　处：《齐鲁医学杂志》2008年第3期231-232,235,共3页Medical Journal of Qilu

摘　　要：目的 探讨细胞周期蛋白（CyclinD1）、细胞周期素依赖性激酶抑制蛋白（P27）在胃癌组织表达及其意义。方法 应用组织微阵列技术结合免疫组织化学方法 （PV-9000法）检测CyclinD1、P27在正常胃黏膜、癌旁组织及胃癌组织中的表达。结果 正常胃黏膜、癌旁组织及胃癌组织中CyclinD1的表达率分别为17.6%、32.7%、65.5%,P27的表达率分别为61.9%、23.9%、13.3%,三组间CyclinD1、P27的表达率比较差异有显著性（H=54.35、46.92,P〈0.01）。CyclinD1、P27在肿瘤中的表达呈负相关（r=-0.209,P〈0.05）。CyclinD1过表达和P27表达下降与胃癌浸润程度和淋巴结转移有关（χ^2=4.86～9.04,P〈0.05）。结论 CyclinD1过表达和P27表达下降在胃癌的发展过程中起重要作用,对早期预测癌细胞的转移潜能、判断胃癌预后有重要的临床意义。Objective To investigate the expression of CyclinD1 and P27 in gastric cancer and their clinical significance. Methods The expression of CyclinD1 and P27 were determinded in normal gastric tissue, the adjacent cancer tissue and gastric cancer with the immunohistochemistry and tissue microarry technique. Results In normal gastric tissue, the adjacent cancer tissue and gastric cancer, positive immuostaining of cyclinD1 were 17.6 %, 32.7 %, 65.5 %, respectively; While positive immuostaining of P27 were 61.9%, 23. 9%, 13.3%, respectively. The expressions of CyclinD1 and P27 were significantly different between the three tissues （ H = 54.35,46. 92 ; P 〈0.01）. Expression of CyclinD1 was negatively correlated with expression of P27 （r = -0. 209,P〈0.05）. The high expression of CyclinD1 and the low expression of P27 were correlated with depth of invasion and lymph node metastasis （Х^2= 4.86-9.04 ,P〈0.05）. Conclusion The results suggest that high expression of CyclinD1 and low expression of P27 may play an important role in the development of gastric cancer and be related to the prognosis of the disease. Tissue microarry is an efficient, low consume and strong comparability technique in the study of human pathology.

关 键 词：胃肿瘤 细胞周期蛋白D1 细胞周期素依赖性激酶抑制蛋白 芯片分析技术 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]