P转位子诱变的果蝇心脏发育基因的突变(Ⅰ)——第2染色体基因突变  

Mutations Affecting Heart Developmental Genes of Drosophila by Single Pelement Insertional Mutagenesis (Ⅰ〗) ——Mutations on Chromosome 2

作　　者：李敏[1] 袁婺州[1] 吴秀山[1] 

机构地区：[1]湖南师范大学生物系

出　　处：《湖南师范大学自然科学学报》1997年第4期66-69,94,共5页Journal of Natural Science of Hunan Normal University

基　　金：湖南省自然科学基金

摘　　要：果蝇和脊椎动物的心脏早期发育具有平行的基因控制机理，果蝇是替代人体心脏进行基因功能研究的理想模式．利用Ｐ转位子作为诱变工具，诱发果蝇第二染色体基因突变，筛选与心脏发育有关的基因．初步结果表明，有３个第二染色体隐性致死突变基因在心脏组织有ｌａｃＺ表达，并且观察到有心脏前体细胞的突变表型．这预示在这３个致死系中，Ｐ转位子可能插入到与心脏发育有关的基因中或这些基因的附近．Since there are parallel genetic principles that operate during early heart development of Drosophila and vertebrates, Drosophila has been proven to be an excellent model system for studying the function and orchestration of heart developmental genes of human being. Here we report that induced gene mutation of Drosophila on chromosome 2 by single Pelement insertional mutagenesis and screened genes relating to heart development. Preliminary experimental results showed that three recessive lethal mutant genes, located on chromosome 2 of Drosophila, could be expressed lac Z protein in heart tissue and observed some mutant phenotypes in heart progenitor cells. It is suggested that pelement may be inserted in the middle or near genes which related to heart development for three lethal systems. We want to further study three lethal systems.

关 键 词：发育 基因 染色体 P转位子 基因突变 心脏病 

分 类 号：R541[医药卫生—心血管疾病]