沙棘果水溶性多糖H_n的分离纯化与抗病毒研究  

Study on Isolation,Purification and Anti-Virus Activity of Polysaccharide from the Fruits of Hippophae rhamnoides L.

作　　者：陈玉香[1,2] 张丽萍[1,2] 梁忠岩[1,2] 苗春艳[1,2] 张翼伸[1,2] 

机构地区：[1]东北师范大学生命科学学院 [2]吉林工业大学食品与包装工程学院

出　　处：《东北师大学报（自然科学版）》1997年第4期74-77,共4页Journal of Northeast Normal University(Natural Science Edition)

摘　　要：沙棘果皮沸水煮提得到粗多糖，经纸层析及气相色谱分析，该粗多糖组成为Ａｒａ，Ｆｕｃ，Ｘｌｙ，Ｇａｌ，Ｍａｎ，Ｇｌｃ和ＧａｌＡ．粗多糖经酸性乙醇分级得四个级分．将对柯萨奇病毒（ＣｏｘｓａｃｋｉｅＢＶｉｒｕｓ，ＣＢＶ）Ｂ３有初步抑制作用的ＨＲ３用ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５柱层析进一步分级，得到２个级分Ｈｎ和Ｈａ．Ｈｎ是由Ａｒａ，Ｇａｌ，Ｍａｎ和Ｇｌｃ组成的中性杂多糖，对柯萨奇病毒Ｂ３具有明显的抑制作用．The crude polysaccharide was isolated by boiling water. PC and GC show it consists of Ara, Fuc, Xly, Gal, Man, Glc and GalA. The crude polysaccharide has been fractionated with acidified ethanol. Four fractions were got respectively. HR 3 has anti-virus activity. HR 3 was further purified by chromatography on a columm(2 5×90 cm) of DEAE-Sephadex A-25. Two fractions were obtained. They are neutral component H n and acidic component H a. H n has notable anti virus (CBV 3) activity.

关 键 词：多糖 柯萨奇病毒B3 抗病毒活性 沙棘果 水溶性 

分 类 号：R282.710.5[医药卫生—中药学] Q949.761.2[医药卫生—中医学]