实验性视网膜脱离时神经生长因子对凋亡相关蛋白表达的影响  

Effects of nerve growth factor on expression of apoptosis-related genes in experimental retinal detachment

作　　者：钱锦[1] 孙晓东[1] 许薇琦[1] 刘海洋[1] 张皙[1] 许迅[1] 陆洪芬[2] 陈荣家[3] 路长林[4] 

机构地区：[1]上海交通大学第一人民医院眼科,上海200080 [2]复旦大学肿瘤医院病理科,上海200032 [3]复旦大学眼耳鼻喉科医院病理科,上海200031 [4]第二军医大学神经生物教研室,上海200433

出　　处：《上海交通大学学报（医学版）》2008年第6期630-634,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30572012);上海市卫生局医学领先专业重点课题(2203)~~

摘　　要：目的研究神经生长因子(NGF)对实验性视网膜脱离(RD)后视网膜细胞凋亡相关基因c-fos、bcl-2、Fas-L与caspase-3表达的影响。方法20只大鼠右眼和左眼分别设为实验组(NGF组)和实验对照组(PBS组),另设立正常对照2只。通过在视网膜下注射透明质酸钠的方法建立视网膜脱离(RD)动物模型后,右眼在玻璃体腔内注射NGF 5μg/次(1 g/L),每4 d注射1次,作为NGF组;左眼注射PBS作为实验对照组。两组在建立模型后1.5、3、6、12 h和1、2、4、8、16、32 d分别取眼球,采用免疫组化学法对视网膜细胞的bc l-2、Fas-L、caspase-3和c-fos进行标记。结果Fas-L、bc l-2、caspase-3和c-fos在实验各组均有表达,并随着时间的变化而变化。NGF组的各基因蛋白表达与PBS组相比差异有统计学意义(P<0.05)。NGF组的c-fos蛋白表达与PBS组相比,表达高峰时间延迟,且表达量下降(P<0.05)。结论实验性RD后给予外源性NGF能促进bc l-2基因的表达,抑制c-fos基因和Fas-L的表达,影响caspase-3的激活和表达,从而抑制RD后视网膜细胞凋亡的发生。Objective To observe the effects of nerve growth factor （NGF） on the expression of apoptosis-related genes such as c-fos, bcl-2, Fas-L and caspase-3 in the retinal cells in experimental retinal detachment （RD）. Methods Twenty rats were selected, and the right and left eyes were served as experimental group （ NGF group ） and experimental control group（PBS group）, respectively. Two rats were selected as the normal control group. After the RD model was set up by subretinal injection with sodium hyaluronate, 5 μL （1 g/L） NGF or 5μL （1 g/L） PBS were intravitreally injected into the right or left eyes, respectively. The injection was performed every 4 d till the end of the observation period. The expressions of bcl-2, Fas-L, caspase-3, and c-fos were detected by immunohistochemistry at 1.5, 3,6, 12 h and 1,2,4,8, 16,32 d time points. Results The expressions of Fas-L, bcl-2, caspase-3 and c-fos were identified and differed between NGF group and PBS group （P 〈 0.05）. The peak of c-fos protein expression was delayed and decreased in NGF group compared with PBS group （P 〈 0.05）. Conclusion Intravitreal injection of exogenous NGF can promote the expressions of bcl-2,inhibit the expressions of c-fos and Fas-L, and influence the expressions of caspase-3. All these result in the suppression of the apoptosis of retinal cells after RD.

关 键 词：视网膜脱离 神经生长因子 凋亡 

分 类 号：R774.1[医药卫生—眼科]