鞘内注射强啡肽A(1-17)对不同功能运动神经元群树突的影响  

Effect of intrathecal administration of dynorphin A(1-17) on the dendrites of two kinds of motoneurons in rat

作　　者：王磊[1] 康云霄[2] 李双成[1] 闫文升[2] 崔慧先[1] 石葛明[2] 

机构地区：[1]河北医科大学解剖学教研室,石家庄050017 [2]河北医科大学神经生物学研究室,石家庄050017

出　　处：《解剖学杂志》2008年第3期362-364,367,共4页Chinese Journal of Anatomy

基　　金：河北省自然科学基金(397254)

摘　　要：目的：探讨强啡肽A对大鼠趾长伸肌（EDL，快肌）、比目鱼肌（SOL，慢肌）两种不同功能运动神经元（Mn）群树突的影响。方法：采用脊髓蛛网膜下隙给予强啡肽A，以霍乱毒素B亚单位结合辣根过氧化酶（CB-HRP）逆行标记EDL-Mn、SOL—Mn群，Mesulam-TMB法显示两运动神经元群被标记的树突。结果：与相应对照组比，强啡肽A致一过性后肢瘫大鼠用药后1h，位于腰4～5脊髓节段腹角的EDL-Mn、SOL-Mn群树突的分布范围减小，尤以EDL-Mn群为甚；EDL-Mn平均树突长度比对照缩短59．5％，而SOL-Mn平均树突长度比对照缩短35．6％。永久性后肢瘫大鼠用药后3h，EDL-Mn群仅见胞体和近端树突；与之相比，SOL-Mn群仍保留有较长的树突和较广的分布范围。结论：强啡肽A致一过性后肢瘫大鼠两群运动神经元树突明显受累，且以EDL-Mn群为甚，其差别可能与两群运动神经元所接受强啡肽A信息传入不同有关。Objective： To study the effect of dynorphin A （Dyn） on the dendrites of two kinds of motoneuron （Mn） pools in rat extensor digitorum longus （EDL, fast-twitch） and soleus （SOL, slow-twitch）. Methods ： Intrathecal administration of Dyn and cholera toxin B subunit conjugated with horseradish peroxidase （CB-HRP） retrograde labeling were mainly used. The dendrites of EDL-Mn or SOL-Mn pools were revealed by Mesulam-TMB and identified by CB-HRP. Results ： Compared with the corresponding control, the scopes of dendrites of EDL-Mn and SOL-Mn pools at the level of the fourth and fifth lumbar segments decreased in Dyn-induced transient paralysis rats, especially in EDL-Mn pool. 1 h after Dyn-treatment, the average dendritic length of Mn in Dyn-induced transient paralysis rats was significantly shorten 59.5 % and 35.6% of the corresponding control in EDL-Mn and SOL-Mn respectively. In Dyn-induced irreversible paralysis rats, 3 h after Dyn-treat- ment, only the perikarya and proximal dendrites left in EDL-Mn pool. In contrast, the more extensive and longer dendrites were still observed in SOL-Mn pool. Conclusion ： The results above indicate that the dendrites of EDL-Mn and SOL-Mn are affected in Dyn-induced transient paralysis rats, especially in EDL-Mn, which may reflect the difference of Dyn inputs to both motoneuron pools.

关 键 词：强啡肽A(1—17) 运动神经元 树突 

分 类 号：R363[医药卫生—病理学]