胰岛素样生长因子-1对雪旺细胞氧化损伤的保护作用  

Protective effects of insulin-like growth factor-1 on the oxidative injuried Schwann cells

作　　者：丛丽[1] 李振华[1] 孙晋浩[1] 暴丽华[1] 卢静[1] 刘文静[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012

出　　处：《解剖学杂志》2008年第3期365-367,共3页Chinese Journal of Anatomy

基　　金：山东省自然科学基金(Y2007C031);山东省优秀中青年科学家科研奖励基金(Y2007C003)

摘　　要：目的:探讨胰岛素样生长因子-1(IGF-1)对雪旺细胞氧化损伤的保护作用。方法:用体外纯化培养的雪旺细胞建立氧化损伤模型,将培养细胞分成氧化损伤组、IGF-1保护组和正常对照组。四甲基偶氮唑蓝比色法(MTT)检测细胞的活性,生化技术检测细胞内超氧化物歧化酶(SOD)的含量,免疫印迹法检测凋亡蛋白Bcl-2的表达水平。结果:与对照组相比,H2O2处理组细胞胞体积缩小、空泡化,细胞活性降低,SOD含量明显减少,Bcl-2表达减弱;而IGF-1保护组细胞存活率明显升高,SOD含量较损伤组高,Bcl-2表达明显上调。结论:IGF-1对氧化损伤的雪旺细胞有保护作用。Objective： To investigate the protective effects of the insulin-like growth factor-1 on the oxidative injured Schwann cells. Methods： Cultured Schwann cells were randomly divided into experimental groups and control groups, the former were subdivided into hydroqen peroxide treated group and insulin-like growth factor-1 preconditioned group. MTT was used to observe the viability of the Schwann cells. The content of intracellular superoxide dismutase （SOD） was detected by a biologic chemistry method. Westem blotting was used to detect the expression of Bcb2. Results： Compared with the control group, the survival Schwann cells decreased in the oxidative injured group. However, these changes were significantly inversed in the insulin-like growth factor-1 preconditioned group. Conclusion： The insulin-like growth factor-1 has a good antioxidant effect on the Schwann cells.

关 键 词：胰岛素样生长因子-1 雪旺细胞 细胞凋亡 BCL-2 

分 类 号：R363[医药卫生—病理学]