两种富硒植物对大鼠高脂血症和脂质过氧化作用的影响  

INFLUENCE OF TWO KINDS OF SELENIUM-ENRICHED PLANTS ON HYPERLIPEMIA AND LIPID PEROXIDATION IN EXPERIMENTAL HYPERLIPEMIC RATS

作　　者：陈玉梅[1] 陈竞[1] 李卫东[1] 毛德倩[1] 杨文婕[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《营养学报》2008年第3期262-265,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30471454)

摘　　要：目的比研究富硒绿羽和富硒蒜抑制高脂血症和脂质过氧化作用效果。方法取Wistar大鼠60只,经适应性喂养1w以后,按照体重队列随机分为6组,分别为基础饲料对照组、高脂饲料对照组、富硒蒜治疗组、富硒绿羽治疗组、富硒蒜预防组和富硒绿羽预防组。除基础饲料对照组外,其余5组均喂饲高脂饲料。富硒植物处理组以每日300μgSe/kgbw植物硒分别灌胃12w(预防组)和4w(治疗组),预防组从第2w、治疗组从第3个月开始植物硒灌胃,基础饲料对照组以同等体积蒸馏水灌胃。3个月后,处死大鼠,测定血浆、红细胞和肝脏丙二醛(MDA)、血清总胆固醇(TC)、甘油三酯TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量以及全血、肝脏谷胱甘肽过氧化物酶(GPX)活性。结果与高脂饲料对照(HFD)组比较,富硒植物处理组大鼠的血清TC、TG、LDL-C含量和动脉粥样硬化指数(AI)下降,HDL-C水平显著升高,预防组大鼠血脂的变化幅度显著大于治疗组。富硒植物处理组全血和肝脏GPX活性显著提高,血浆、红细胞和肝脏的MDA含量变化复杂,以富硒蒜治疗组MDA含量降低最明显。结论富硒绿羽和富硒蒜具有控制高血脂症发生发展能力,预防效果优于治疗效果。短期摄入富硒蒜清除组织MDA能力最高。Objective the inhibitive efficacies of The hyperlipidemia rat model induced by high-fat diet was applied to compare selenium-enriched garlic and kavas on hyperlipemia and lipid peroxidation Method Sixty adult male Wistar rats were fed the basal diet for a week, and divided equally into the control （CK）, high-fat diet group （HFD）, HFD＋selenium-enriched garlic preventive group （HFD＋SeGP）, HFD＋selenium-enriched green kavas preventive group （HFD＋SeKP）, HFD＋selenium-enriched garlic therapeutic group（HFD＋SeGT） and HFD＋selenium-enriched green kavas therapeutic group （HFD＋ SeKT）. Rats were given 300μg Se/kg bw of daily suspension of Se-enriched plants by gavage for 3 months in two selenium-enriched plant preventive groups, and for the 3rd month in two selenium-enriched plant therapeutic groups. Rats were sacrificed at the end of 3 month, the total cholesterol （TC）, triglyceride （TG）, low density lipoprotein cholesterol （LDL-C）, high density lipoprotein cholesterol （HDL-C） in serum, the maleic dialdehvde （MDA） in olasma, red cell and liver and the activities of glutathione oeroxidase （GPX） in liver and blood were determined. Results The serum concentrations of TC, TG and LDL-C in rats supplemented by selenium-enriched plants were significantly lower than those in HFD group. The serum HDL-c and the blood and liver GPX activities in rats supplemented by selenium-enriched plants were significantly higher than those in HFD group, and the changes of the preventive groups were significantly much more than those of the therapeutic groups. The lowest MDA level occurred in rats in the selenium-enriched garlic therapeutic group. Conclusion Selenium-enriched garlic and selenium-enriched green kavas inhibit the development of hyperlipemia, and the preventive efficacy is significantly better than the therapeutic one. The selenium-enriched garlic supplementation for 1 month shows the highest efficacy in the elimination of rat blood and liver MDA.

关 键 词：富硒植物 高血脂症 脂质过氧化 

分 类 号：R285.5[医药卫生—中药学]