龙柚芽变系的RAPD分析鉴定  

RAPD Studies on Sport Lines of Citrus grandis Osbeck cv.Long Yu

作　　者：朱旸[1] 潘一山[1] 郑少缘[2] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000 [2]华南农业大学林学院,广东广州510642

出　　处：《漳州师范学院学报（自然科学版）》2008年第2期99-101,共3页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：福建省自然科学基金(2006J0382);福建省科技计划项目(2006N0045);福建省教育厅资助科技项目(JB07146)

摘　　要：利用100个10bp的随机引物对龙柚的4个芽变系的基因组DNA进行RAPD扩增分析,探讨芽变系遗传物质的变化.在这100个随机引物中,筛选出能在4个芽变系中扩增出稳定多态性片段的引物11个,一共扩增出了63条片段,其中多态性片段有19个,占30.16%.聚类分析的结果,芽变系2和4的遗传距离最小(0.1001),相似系数最大(0.9048).表明了芽变引起的遗传物质的改变能够通过无性繁殖的方式存在,用RAPD的方法来分析不同芽变系的遗传关系是可行的.Genetic DNA of four sport lines from Citrus grandis Osbeck cv.Long Yu were amplifide by RAPD with 100 10bp random primers to study the genetic substance of them. 11 primers were screende which could produce clear and stability polymorphic DNA fragments. 19 out of the total 63 fragments amplified were distinctive including the loss of a nomal segment and appearance of a new one. Further studying showed that the genotype of the sport could be inherited by way of asexual propagation.

关 键 词：龙柚 芽变 随机扩增多态性DNA(RAPD) 

分 类 号：S666.3[农业科学—果树学]