肺癌甲状腺转录因子-1的表达及其与细胞凋亡和血管新生关系探讨  

Expression of Thyroid Transcription Factor-1 and Its Correlation with Apoptosis and Angiogenesis in Lung Cancer

作　　者：白晓燕[1] 申洪[1] 周春辉[1] 王灏[1] 

机构地区：[1]南方医科大学基础医学院病理学教研室,广州市510515

出　　处：《中国肿瘤临床》2008年第12期693-696,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30271462);广东省科技攻关计划基金(编号:2KM04501S);广州市科技攻关重点基金资助(编号:2003Z2-E0061;E0062)~~

摘　　要：目的:探讨肺癌甲状腺转录因子-1(TTF-1)表达与细胞凋亡和血管新生的关系。方法:构建含196例肺癌组织、10例正常肺组织和1例肌肉组织的829点阵的石蜡组织芯片。用原位末端转移酶标记检测法(TUNEL)和免疫组化SP法,检测细胞凋亡及TTF-1和CD34的表达。应用LeicaQ500MC图像分析系统测试TTF-1阳性单位值(Positive Unit,PU)、细胞凋亡指数(Apoptotic Index,AI)和微血管密度(Microvessel Density,MVD)。结果:肺小细胞癌和大细胞癌AI小于肺腺癌和鳞癌,差异有显著性(P=0.000)。有淋巴结转移组AI小于无淋巴结转移组,差异有显著性(P=0.039);TNMⅡ～Ⅳ期组AI小于Ⅰ期组,差异有显著性(P=0.008)。肺小细胞癌TTF-1的PU值与AI呈负相关(r=-0.752,P=0.000)。无淋巴结转移组MVD小于有淋巴结转移组(P=0.031);TNMⅠ期组MVD小于Ⅱ～Ⅳ期组,差异有显著性(P=0.040)。肺腺癌TTF-1的PU值与MVD呈正相关(r=0.708,P=0.000)。结论:肺小细胞癌TTF-1的PU值与AI呈负相关,二者之间可能存在一定相互作用关系。肺腺癌TTF-1的PU值与MVD呈正相关,TTF-1可能通过促进肿瘤微血管生成促进肺腺癌的发生、发展。Objective： To investigate the correlation between the expression of thyroid transcription factor-1（TTF-1） and apoptosis and angiogenesis in lung cancer. Methods： A paraffin tissue microarray containing 829 tissue cores including 196 lung carcinomas, 10 normal lung tissues and 1 muscular tissue was constructed. Terminal deoxynucleotidyl transferase dUTP nick end labeling（TUNEL） and immunohistochemical staining were used to detect apoptosis and the expression of TTF-1 and CD34 in different types of lung carcinomas. A Leica Q500 MC image analysis system was employed to measure and calculate TTF-1 Positive Units （PU）, Apoptotic Index（AI）, and Microvessel Density（MVD）. Results： AI of small cell lung cancer and large cell lung cancer was lower than that of adenocarcinoma and squamous cell carcinoma of the lung（P=0.000）. AI of lung carcinomas with lymph node metastases was lower than that of those without lymph node metastases（P=0.039）. AI of lung carcinoma in TNM stage Ⅱ-Ⅳ was lower than that in stage Ⅰ （P=0.008）. TTF-1 PU was negatively correlated with AI in small cell lung cancer（r=-0.752,P=0.000）. MVD of lung carcinomas without lymph node metastases was lower than that of those without lymph node metastases（P=0.031）. MVD of lung carcinomas in TNM stage Ⅰwas lower than that in stage Ⅱ-Ⅳ（P=0.040）. TTF-1 PU was positively correlated with MVD in lung adenocarcinoma（r=0.708,P=0.000）. Conclusion： There is a negative correlation between TTF-1 PU and AI in small cell lung cancer. TTF-1 PU was positively correlated with MVD in lung adenocarcinoma. TTF-1 may contribute to the development of lung adenocarcinoma by inducing tumor angiogenesis.

关 键 词：肺癌 甲状腺转录因子-1 组织芯片 细胞凋亡 血管新生 

分 类 号：R734.2[医药卫生—肿瘤] R735.2[医药卫生—临床医学]