南方鲶SME-1细胞系、草鱼CIK细胞系与原鱼种的RAPD图谱比较  

The RAPD comparation among SME 1 cell line,CIK cell line,and their original fish

作　　者：崔志鸿[1,2] 洪锡钧[1,2] 裴炎[1,2] 

机构地区：[1]西南师范大学生命科学系 [2]西南农业大学生物技术中心

出　　处：《西南师范大学学报（自然科学版）》1997年第6期665-670,共6页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：研究了ＳＭＥ１和ＣＩＫ细胞系，以及南方鲶、鲶、大鳍、草鱼等６个样品的ＲＡＰＤ图谱，测定了各样品间的Ｎｅｉ相似系数和单匹配相似系数，并绘制了聚类树形图．Ｎｅｉ相似系数更适于细胞系与原种动物的相似性分析；细胞系与原鱼种的相似系数大于种间相似系数；培养时间短的ＳＭＥ１细胞与其原鱼种南方鲶的相似系数大于培养时间长的ＣＩＫ细胞与其原生鱼种草鱼的相似系数．Random Amplified Polymorphic DNA(RAPD) analysis was applied to two cell line:SME 1 and CIK,three species of the catfish: S astotus linnacus,S soldatovi merionalis Chen,and M.macropterus Bleeker and C idellus .The RAPD patterns were compared by Nei similarity and simple matching coefficients of similarity,and the dendrograms were drawn.The Nei similarity was more useful in the comparision between cell line and their original fish.The similarity between cell line and their original fish was higher than that between species.The similarity between the short time cultured cell line and its original fish was higher than that between the long time cultured cell line and its original fish.

关 键 词：细胞系 RAPD 南方鲶 草鱼 SME-1 CIK 

分 类 号：Q959.468[生物学—动物学]