染砷大鼠睾丸生精细胞凋亡的变化  

Study of As_2O_3 on Apoptosis of Spermatogenic Cells of Rat Testis

作　　者：陆祥[1] 陈伟[1] 舒小林[1] 

机构地区：[1]遵义医学院组织与胚胎学教研室,贵州遵义563003

出　　处：《四川解剖学杂志》2008年第2期11-14,共4页Sichuan Journal of Anatomy

基　　金：贵州省科技厅国际合作重点项目(C-293)

摘　　要：目的观察不同剂量的三氧化二砷(As2O3)对成年大鼠生精细胞凋亡的影响。方法40只健康雄性SD大鼠随机分成4组,分别为0.375 mg/kg、0.75 mg/kg、1.5 mg/kg 3个染毒组和对照组,灌胃法连续给药16周后对各组大鼠进行睾丸脏器系数、精子头计数,并计算每日精子生成量(DSP)。采用甲基绿—派诺宁染色法和TUNEL原位细胞杂交方法检测大鼠生精细胞凋亡情况。结果①中剂量组(0.75 mg/kg)和高剂量组(1.5 mg/kg)睾丸精子头计数和DSP均低于对照组(P<0.05);②与对照组相比,中剂量组和高剂量组生精细胞凋亡指数(AI)均显著升高(P<0.01);③生精细胞凋亡指数与每日精子生成量呈负相关(r=-0.563,P<0.01)。结论一定剂量的As2O3可通过促进生精细胞凋亡而导致精子生成量的减少,产生生殖毒性。Objective To investigate the effect on the apoptosis of spermatogenic cells in adult rats with different doses of arsenic （As2O3）. Methods 40 healthy male Sprague-Dawle rats were divided randomly into four groups and they were administered respectively with 0 （control group）, 0. 375, 0. 75 and 1.5 mg · kg^-1 body weight of As2O3 by intragastric administration consecutively for 16 weeks. The numbers of testicular sperm head were counted and the coefficient of testicular viscera , daily sperm production was calculated in every group. The apoptosis of germ cell were assessed by in situ terminal deoxynucleotityl transferase mediated dTUP nick end labeling（TUNEL） technique and methyl green-pyronin staining method. Results ①In the testes of As2O3-treated rats,The coefficient of testicular viscera , the number of testicular sperm head and daily sperm production in mid and large-dose groups decreased significantly than that in the control（P〈0. 05） ;② the apoptosis index of germ cell（AI） significantly increased compared with that in the control group （P〈0. 01） ;③There were negative correlation between the apoptosis index of germ cell（AI） and the content of serum arsenic （r=-0. 563,P〈0.01）. Conclusions The apoptosis of spermatogenic cells can be aggravated after As2O3 administration in male rats, it may reduce the sperm production and lead to the reproduction toxicity.

关 键 词：AS2O3 细胞凋亡 生精细胞 大鼠 

分 类 号：R363[医药卫生—病理学]