表面增强激光解吸/离子化蛋白质芯片技术在肝细胞癌早期诊断研究中的应用  

Application of Surface Enhanced Laser Desorption Ionization Proteinchip Technology in Diagnosis of Early Hepatocellular Carcinoma

作　　者：吴飞翔[1] 王琪[2] 

机构地区：[1]广西医科大学附属肿瘤医院肝胆乳腺科,南宁530021 [2]广西医科大学附属肿瘤医院实验研究部,南宁530021

出　　处：《医学综述》2008年第13期1963-1966,共4页Medical Recapitulate

基　　金：广西青年科学基金资助项目(桂科青0447050)

摘　　要：随着蛋白质组学研究技术的飞速发展,集样品分离、纯化、检测和数据分析为一体的表面增强激光解吸离子化飞行时间质谱蛋白质芯片技术已成为蛋白质组学研究的有力工具,它可以快速、简便地直接检测各种体液及分泌物,具有标本用量少、高通量分析等特点,并在筛选、鉴定肿瘤标志物及临床疾病的诊断、防治方面发挥了重要作用。本文介绍了该技术的特点和分析步骤,以及该技术在肝细胞癌早期诊断中的应用情况,并对其前景进行展望。With the rapid development of the research technique for proteomics, the protein chip technology of surface enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS） has become a powerful tool in the research field of proteomies, which set separation, purification, detection and data analyses at the one machine and detect various samples rapidly and sensitively with little specimens and high-throughput ability. SELDI-TOF-MS has been demonstrated to play an important role in selection and identification of tumor markers, and in diagnosis, prevention and treatment of clinical diseases. This article summarized characteristics and analysis procedure of SELDI-TOF-MS, its application in early diagnosis of hepatecellular carcinoma, and development prospects of this technique.

关 键 词：蛋白质芯片 表面增强激光解吸/离子化飞行时间质谱 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]