外源性视黄酸调节胚鼠心肌TBX1基因表达的研究  

The influence of exogenous retioic acid on the TBX1 mRNA expression in embryonic mouse myocardial cell

作　　者：刘苗[1] 吴小燕[1] 徐佳伟[1] 金润铭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院儿科

出　　处：《华中医学杂志》2008年第3期158-160,共3页Central China Medical Journal

基　　金：国家自然科学基金资助项目(No30400484)

摘　　要：目的观察外源性视黄酸(RA)对胚鼠心肌细胞TBX1基因mRNA水平表达的影响。方法分离培养胚鼠心肌细胞,经不同浓度的RA处理后,采用RT-PCR方法分别检测胚鼠心肌细胞TBX1基因在mRNA水平表达有无变化。结果①TBX1基因在胚鼠心肌细胞中有基础表达,在予以RA刺激后,1×10-8mol/LRA和1×10-7mol/LRA使细胞内TBX1基因mRNA表达分别增加54.94%和89.53%,而1×10-6mol/LRA使TBX1基因mRNA表达降低47.94%。②在最适浓度条件下(1×10-7mol/LRA),在予以RA刺激8h、12h、16h后TBX1基因mRNA表达水平分别增加1.65倍、1.35倍和1.04倍,差异有显著意义。结论外源性视黄酸在一定浓度下可以促进胚鼠心肌TBX1基因mRNA的表达,为进一步研究先天性心脏病的遗传基础提供了依据。Objective To observe the influence of exogenous retioic acid on the TBX1 mRNA expression in embryonic mouse myocardial cell. Methods We measured the expression of TBX1 mRNA by using RT-PCR technique, and detected the best time to intervene. Results ①There is basal expression of TBX1 in the control group. When treated with 1 × 10^-8 mol/L and 1 × 10^-7mol/L RA, we observed that the expression of TBX1 mRNA in embryonic mouse myocardial cell had an increase of 54. 94% and 89. 53% in comparison with control group respectively. However, at the concentration of 1 × 10^-6 mol/L RA, it resulted in 47. 94% decrease than the control group. ②While exposed to the optimum concentration （1 × 10^-7 mol/L RA）, after 8hr, 12 hr and 16 hr treatment with RA, it showed that TBX1 mRNA expression had an increase ot＂ 1.65-fold, 1.35-fold and 1.04-fold in comparison with control group respectively. Conclusion Exogenous RA could promote embryonic mouse cardio- myocytes＂ TBX1 mRNA expression in some concentration. This may provide a new basis for genetic research of congenital heart disease.

关 键 词：视黄酸 TBX1基因 心肌细胞 

分 类 号：R541[医药卫生—心血管疾病] R542.2[医药卫生—内科学]