海带褐藻糖胶磺化工艺研究  

Study on the Preparation of Sulfated Fucoidan Extracted from Laminaria japonica

作　　者：王晶[1] 张忠山[1] 张全斌[1] 胡如桂[1] 綦慧敏[1] 李智恩[1] 徐祖洪[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《时珍国医国药》2008年第6期1386-1387,共2页Lishizhen Medicine and Materia Medica Research

基　　金：中国科学院知识创新工程重要方向项目(No.KZCX2-YW-209)

摘　　要：目的优选海带褐藻糖胶(FPS)磺化的工艺。方法以氯磺酸-N,N-二甲基甲酰胺(DMF)为磺化试剂,采用正交设计法,考察反应时间、反应温度及磺化剂用量对海带褐藻糖胶磺化反应的影响,找出制备高硫酸根含量海带褐藻糖胶的最佳方法。结果反应时间和反应温度对海带褐藻糖胶的磺化有显著影响(0.01<P<0.05),磺化剂用量对海带褐藻糖胶的磺化有一定影响(0.05<P<0.1)。实验得到的海带褐藻糖胶的最佳磺化工艺为:反应时间3 h,反应温度50℃,磺化剂用量25 m l。结论用SO3-DMF作为磺化试剂对海带褐藻糖胶进行磺化是可行的,可避免使用毒性较强的SO3-吡啶作为磺化试剂,方法简单,可用于制备保健品和药品。Objective To explore the optimum method for preparation of sulfated fucoidan extracted from Laminaria japonica. Methods Using chlorsulfonic acid and DMF as sulfated reagent, test the effect of reaction time, reaction temperature and volumn of sulfated reagent by the orthogonal design method. Results Reaction time and reaction temperature were the significant factors （0.01 〈 P 〈 0.05）. Volumn of sulfated reagent had some effects on the sulfation reaction （0.05 〈 P 〈 0.1 ）. The best process was set as follows： the reaction time was 3 h at 50℃ with 25 ml sulfated reagent. Conclusion Reaction time and reaction temperature are the significant factors （0.01 〈 P 〈 0.05 ）. Volumn of sulfated reagent has some effects on the sulfation reaction （ 0.05 〈 P 〈0.1 ）. The best process is set as follows ： the reaction time is 3 h at 50℃with 25 ml sulfated reagent.

关 键 词：海带 褐藻糖胶 磺化 硫酸根 

分 类 号：R283[医药卫生—中药学]