日本血吸虫生殖相关抗原31～32kDa分子的质谱分析与鉴定  

Identification and analysis of effective compositions of Schistosoma japonicum 31-32 kDa proteins

作　　者：李林[1] 汪世平[1] 周帅锋[1] 胡少敏[2] 何卓[1] 高冬梅[1] 冯明钊[2] 

机构地区：[1]中南大学湘雅医学院,长沙410078 [2]香港中文大学

出　　处：《中国血吸虫病防治杂志》2008年第3期168-173,共6页Chinese Journal of Schistosomiasis Control

基　　金：国家重点基础研究发展计划资助(973)(2007CB513108);湖南省“十一五”重大专项(2006SK1001);湖南省重点学科建设专项经费(07-985-2)

摘　　要：目的利用蛋白质组学技术分离、鉴定日本血吸虫天然分子31～32kDa抗原组分。方法收集感染后32d的日本血吸虫成虫,制备成虫蛋白,经一维电泳、固相pH梯度双向凝胶电泳分离,选取31～32kDa附近的蛋白点进行质谱分析鉴定,通过凝胶图像分析软件进行结果比较。结果二维电泳结果显示31～32kDa附近有13个蛋白点,经MALDI-TOF-MS鉴定及分析,发现3个蛋白分子分别与曼氏血吸虫Actine-2、日本血吸虫3-磷酸甘油脱氢酶、曼氏血吸虫组织蛋白酶B肽链内切酶有高度的同源性。结论31～32kDa组分中含有这3个蛋白点,它们与日本血吸虫31～32kDa天然分子中起保护性的有效分子密切相关。同时,它们的发现也为天然分子疫苗向基因工程疫苗的推进奠定了基础。Objective To identify and analyze the effective compositions of Schistosoma japonicum 31-32 kDa proteins by using the techniques of proteomics. Methods The total proteins were prepared from 32-day adult worms of Schistosoma japonicum. After two-dimensional （2-D） gel elec- trophoresis, the distinct protein spots from 2-D gels were isolated and analyzed by MALDI-TOF-MS. Results A total of 13 protein spots, within the range of 31-32 kDa, were detected in the 2-D gels. Three of them had high homology with Actine-2 of S. mansoni, glycerol-3-phosphate dehydrogenase of S. japonicum and cathepsin B endopeptidase of S. mansoni. Conclusions The 31- 32 kDa antigens contain 3 important antigens ： actine-2, glycerol-3-phosphate dehydrogenase and cathepsin B endopeptidase, which have been demonstrated to have certain protective effect against S. japonicum. Our findings can facilitate the development of multi-epitope vaccine against S. japonicum.

关 键 词：日本血吸虫 疫苗 蛋白质组学 二维电泳 质谱 

分 类 号：R383.24[医药卫生—医学寄生虫学]