DL-ATC对TS1138菌株酶法生产L-半胱氨酸的影响  

Effect of DL-ATC on the Production of L-Cysteine with Enzymatic Method by Pseudomonas sp.TS1138

作　　者：李梅[1] 黄磊[1] 怀利华[1] 陈宁[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300457

出　　处：《天津科技大学学报》2008年第2期27-29,共3页Journal of Tianjin University of Science & Technology

基　　金：天津市科委应用基础研究重点基金资助项目(05YFJZJC00901)

摘　　要：以假单胞菌(Pseudomonas sp.)TS1138菌株为供试菌株,研究了在转化DL-ATC生产L-半胱氨酸过程中,DL-ATC对产酶和转化的影响.结果表明:DL-ATC对TS1138菌株产酶具有诱导作用,产酶培养中必须适量添加.从L-半胱氨酸的产量和DL-ATC转化率两个方面综合考虑,酶法生产L-半胱氨酸的最适DL-ATC为9 g.L-1,采用间歇流加DL-ATC方式可使L-半胱氨酸的产量提高56.25%.L-cysteine is an elementary S-containing amino acid, which has been widely used in medicines, food additives, and cosmetics.Effect of DL-ATC on the conditions of enzyme production process by Pseudomonas sp.TS 1138 and enzymatic transformation of L-cysteine was discussed.The results show that DL-ATC add in the medium has an inducible impact on enzyme production.On account of the interaction of the L-cysteine yield and conversion ratio of DL-ATC, the optimal concentration of DL-ATC is confirmed at about 9g.L^-1.The L-cysteine yield is increased by 56.25% with the DL-ATC interval feeding method.

关 键 词：DL-ATC 酶活力 L-半胱氨酸 

分 类 号：Q93[生物学—微生物学]